Novel chimaeric protein expressed in Philadelphia positive acute lymphoblastic leukaemia
Novel chimaeric protein expressed in Philadelphia positive acute lymphoblastic leukaemia
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费城阳性急性淋巴细胞白血病中表达的新型嵌合蛋白
DOI：
10.1038/329851a0
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发表时间：
1987
期刊：
影响因子：
64.8
通讯作者：
Bryan D. Young
中科院分区：
文献类型：
--
作者：
Lesley C. Walker;T. S. Ganesan;S. Dhut;B. Gibbons;T. Andrew Lister;J. Rothbard;Bryan D. Young
细胞遗传学改变对于理解人类恶性肿瘤的发病机制正变得越来越重要。T(9；22)(q34；qL1)易位是最一致的易位之一，并产生费城染色体(PH1)(参考文献1)。1)在慢性粒细胞白血病(CML)中；在一些急性淋巴细胞性白血病(ALL)中也观察到了它。2)。在慢性粒细胞白血病中，断裂点发生在22号染色体上指定为BCR(参考文献)的区域3)并表达了相对分子质量(MT)为210,000(210K)的bcr-abl融合产物与体外相关的酪氨酸激酶活性4(p210bcr-abl)。在一些PH1阳性的ALL病例中，一种新的190K的ABL相关蛋白(P190all-ABL)被证明具有酪氨酸激酶活性。在这份报告中，我们证明了Pl90all-ABL蛋白具有来自氨基末端的BCR决定簇，但缺乏通常在靠近BCR-ABL连接的P210bcr-ABL中存在的BCR决定簇。用抗ABL和BCR多肽的抗血清进行连续免疫沉淀，证实了P190all-ABL的嵌合体性质。
Cytogenic changes are becoming increasingly important in understanding the pathogenesis of human malignancies. The t(9;22) (q34 ;ql1) translocation is one of the most consistent and generates the Philadelphia chromosome (Ph1) (ref. 1) in chronic myeloid leukaemia (CML); it has also been observed in some acute lymphoblastic leukaemias (ALL) (ref. 2). In CML the breakpoints occur on chromosome 22 in the region designated bcr (ref. 3) and result in the expression of a bcr–abl fusion product of relative molecular mass (MT) 210,000 (210K) with associated in vitro tyrosine kinase activity4 (P210bcr–abl). In some cases of Ph1-positive ALL, a novel abl-related protein (P190all–abl) of 190K has been shown5–7 to have tyrosine kinase activity. In this report we demonstrate that the Pl90all–abl protein has a bcr determinant from the amino-terminal region, but is lacking a bcr determinant normally found in the P210bcr–abl near the bcr-abl junction. The chimaeric nature of the P190all–abl was confirmed by sequential immunoprecipitation with antisera against abl and bcr peptides.
DOI：
10.1073/pnas.83.6.1807
发表时间：
1986-03
影响因子：
11.1
作者：
J. Erikson;Constance A. GRIFFINt;A. Ar-rushdi;M. Valtieri;James Hoxiem;Janet FINANt;Beverly S. EMANUELt-Beverly-S
通讯作者：
J. Erikson;Constance A. GRIFFINt;A. Ar-rushdi;M. Valtieri;James Hoxiem;Janet FINANt;Beverly S. EMANUELt-Beverly-S
慢性粒细胞白血病患者的 bcr-abl RNA。
影响因子：
20.3
作者：
Shtivelman,E;Gale,RP;Dreazen,O;Berrebi,A;Zaizov,R;Kubonishi,I;Miyoshi,I;Canaani,E
通讯作者：
Canaani,E