RNA sequencing analysis of the CAL-27 cell response to over-expressed ZNF750 gene revealed an extensive regulation on cell cycle.
RNA sequencing analysis of the CAL-27 cell response to over-expressed ZNF750 gene revealed an extensive regulation on cell cycle.
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对过表达 ZNF750 基因的 CAL-27 细胞反应的 RNA 测序分析揭示了对细胞周期的广泛调节。
DOI：
10.1016/j.biopha.2019.109377
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发表时间：
2019-10
影响因子：
7.5
通讯作者：
Chen Haiying
中科院分区：
文献类型：
--
作者：
Liu Xianbin;Yang Yikun;Xu Cong;Yang Hongli;Chen Shuangfeng;Chen Haiying
锌指蛋白750（ZNF 750）是口腔鳞状细胞癌（OSCC）的潜在抑癌基因。然而，其抗口腔鳞癌作用的分子机制仍不清楚。本研究旨在通过基因组学方法研究ZNF 750在口腔鳞癌细胞系CAL-27中过表达后的抑瘤作用，探讨ZNF 750在口腔鳞癌细胞系CAL-27中过表达后抑瘤作用的相关基因和途径。采用QPCR和western-blot方法验证细胞周期调控候选基因的差异表达。结果经RNA测序分析，ZNF 750组中有7 131个基因差异表达。在DEG中，根据GO分类法，共发现3,285个表达上调的基因，3,846个表达下调的基因，4,507个表达下调的基因，分为细胞组分、生物学过程和分子功能3大类。京都基因和基因组百科全书（KEGG）通路分析将DEG分为280条通路，并确定了参与剪接体和细胞周期的前两条最重要的通路。功能分类和富集分析显示，ZNF 750过表达后，DEG主要参与结合和催化活性，细胞周期相关基因显著富集。ZNF 750可使CAL-27细胞周期阻滞于G 0/G1期。结论细胞周期调控通路是ZNF 750抗CAL-27肿瘤作用的关键因素。
BackgroundZinc-finger protein 750 (ZNF750) is a potential tumor suppressor in oral squamous cell carcinoma (OSCC). However, the molecular mechanisms underlying its anti-tumor effect remain elusive in OSCC. This study aimed to elucidate the genes and pathways involved in tumor suppression following the ZNF750 over-expression in OSCC cell line CAL-27 cell by using the genomics approach.MethodsThe RNA sequence libraries were constructed, and the data were analyzed to identify differentially expressed genes (DEGs) between vector groups and ZNF750 groups (over-expressed ZNF750 in CAL-27 cell). QPCR and western-blot was used to validate differential expression of candidate genes with cell cycle regulation. The cell cycle distribution was analyzed by BrdU staining.ResultsBy RNA sequencing profiling, 7,131 genes were differentially expressed in ZNF750 groups. Among the DEGs, 3,285 genes were upregulated, 3,846 genes were downregulated and 4,507 genes were identified in three main categories (cellular_component, biological process and molecular function) based on the gene ontology (GO) classification. The Kyoto Encyclopedia of Genes and Genome (KEGG) pathway analysis defined the DEGs could be categorized into 280 pathways and identified the top two most significant pathways involved in spliceosome and cell cycle. Functional categorization and enrichment analysis revealed that most of DEGs involved in binding and catalytic activity, and the cell cycle associated genes were significantly enriched in response to ZNF750 over-expression. ZNF750 induced cell cycle arrest in G0/G1 phase of the cell cycle.ConclusionData from this study revealed that the cell cycle pathway was a key factor involved in the anti-tumor effect of ZNF750 in CAL-27 cells.
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影响因子：
5
作者：
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通讯作者：
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作者：
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通讯作者：
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影响因子：
11.8
作者：
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