Comparison of regression and fat-free diet methods for estimating ileal and total tract endogenous losses and digestibility of fat and fatty acids in growing pigs.
Comparison of regression and fat-free diet methods for estimating ileal and total tract endogenous losses and digestibility of fat and fatty acids in growing pigs.
复制标题
用于估计生长猪回肠和总道内源性损失以及脂肪和脂肪酸消化率的回归法和无脂日粮法的比较。
DOI：
10.1093/jas/skaa376
复制
发表时间：
2020
影响因子：
3.3
通讯作者：
C. Lai
中科院分区：
文献类型：
--
作者：
Lu Wang;L. Wang;Junyan Zhou;Tian;Xiaoxiao Liang;Q. Hu;Bingbing Huang;Z. Lyu;L. Johnston;C. Lai
进行实验以1）比较用于估计生长猪中脂肪和脂肪酸的总或基础内源性损失（ELF）和脂肪酸（ELFA）以及脂肪和脂肪酸的真消化率（TD）或标准化消化率（SD）的回归和无脂肪饮食方法，以及2）比较回肠末端和整个肠道的这些估计值。将10头公猪（初始体重：45.1 ± 2.8 kg）在回肠远端手术安装T型插管，并在3阶段Youden Square设计中分配到5种实验饲料中的1种。用玉米淀粉、大豆分离蛋白和蔗糖配制无脂饲料。在基础日粮中添加2%、4%、6%和8%的大豆油，以玉米淀粉为代价，配制4种不同水平的添加油脂日粮。所有日粮均含有26%甜菜渣和0.40%氧化铬。结果表明，大豆油对脂肪的回肠真消化率（TID）和全消化道真消化率（TTTD）无显著影响。C18：0和总饱和脂肪酸TID均大于TTTD（P < 0.05）。回肠末端的总ELF与整个肠道的ELF没有差异。此外，C18：0和总饱和脂肪酸的总内源性损失在整个肠道比在回肠末端更大，而C18：2和总不饱和脂肪酸的总内源性损失正好相反。对于基础ELF和ELFA观察到类似的结果。随着大豆油添加量的增加，除C18：0的回肠表观消化率外，其余脂肪和脂肪酸的表观消化率均呈线性增加（P < 0.05）。而大豆油的包合量对脂肪和脂肪酸的SD无明显影响。当在回肠末端或总肠道末端测量时，通过回归和无脂饮食方法观察到的ELF和ELFA估计值没有差异。大豆油脂肪和脂肪酸的TD和SD估计值之间无差异。总的来说，可以在整个肠道内测量脂肪的TD或SD的估计值，而回肠分析方法应用于确定脂肪酸的ELF、ELFA和TD或SD。对基础ELF和ELFA表观消化率进行校正，可以准确估计脂肪和脂肪酸的SD值。
An experiment was conducted to 1) compare the regression and fat-free diet methods for estimating total or basal endogenous losses of fat (ELF) and fatty acids (ELFA) and true digestibility (TD) or standardized digestibility (SD) of fat and fatty acids in growing pigs and 2) compare these estimated values at the end of the ileum and over the entire intestinal tract. Ten barrows (initial body weight: 45.1 ± 2.8 kg) were surgically fitted with a T-cannula in the distal ileum, and allotted to 1 of 5 experimental diets in a 3-period Youden Square design. A fat-free diet was formulated using cornstarch, soy protein isolate and sucrose. Four oil-added diets were formulated by adding 4 levels of soybean oil (2, 4, 6 and 8%) to the fat-free basal diet at the expense of cornstarch. All diets contained 26% sugar beet pulp and 0.40% chromic oxide. Results indicated that here were no differences between true ileal digestibility (TID) of fat and true total tract digestibility (TTTD) of fat when pigs were fed soybean oil. The TID of C18:0 and total saturated fatty acids were greater than TTTD (P < 0.05). The total ELF at the end of the ileum was not different from that over the entire intestinal tract. In addition, total endogenous losses of C18:0 and total saturated fatty acids were greater for the entire intestinal tract than at the end of the ileum, whereas total endogenous losses of C18:2 and total unsaturated fatty acids were just the opposite. Similar results were observed for basal ELF and ELFA. As inclusion level of soybean oil increased, apparent digestibility of fat and fatty acids increased linearly (P < 0.05) except for apparent ileal digestibility of C18:0. However, SD of fat and fatty acids were not influenced by inclusion level of soybean oil. Estimation of ELF and ELFA observed by the regression and fat-free diet methods did not differ when measured at the end of the ileal or total tract. There were no differences between estimations of TD and SD of fat and fatty acids for soybean oil. Collectively, estimation of TD or SD of fat can be measured over the entire intestinal tract, whereas the ileal analysis method should be used to determine the ELF, ELFA and TD or SD of fatty acids. Correcting apparent digestibility for basal ELF and ELFA can accurately estimate SD values of fat and fatty acids.
影响因子：
3.5
作者：
VANSOEST, PJ;ROBERTSON, JB;LEWIS, BA
通讯作者：
LEWIS, BA