INDUCTION OF APOPTOSIS IN CERVIX NEOPLASMS HeLa CELLS BY A RAPESEED PEPTIDE HYDROLYSATE FRACTION
INDUCTION OF APOPTOSIS IN CERVIX NEOPLASMS HeLa CELLS BY A RAPESEED PEPTIDE HYDROLYSATE FRACTION
复制标题
油菜籽肽水解产物组分诱导宫颈肿瘤 HeLa 细胞凋亡
DOI：
10.1111/j.1745-4514.2010.00451.x
复制
发表时间：
2011
影响因子：
4
通讯作者：
Jianying Wang
中科院分区：
文献类型：
--
作者：
Zihan Xue;W. Yu;Zhiwei Liu;Moucheng Wu;Jianying Wang
以菜籽粕为原料，经酶解、Sephadex凝胶过滤分离，得到菜籽肽组分（RSP 2）。评价RSP 2对人宫颈HeLa细胞系的抗癌作用。RSP 2处理后HeLa细胞的存活率明显下降，并呈剂量依赖性，细胞形态学改变和凋亡泡现象明显。DNA片段化分析和单细胞凝胶电泳结果显示，RSP 2在各浓度下均能诱导HeLa细胞凋亡，且呈剂量依赖性。结合我们最近发表的数据，RSP 2可以将HeLa细胞在G 0/G1期的百分比从55%降低到43%，并将凋亡细胞的比例从2.9%增加到40%，本研究的结果突出了RSP 2作为对人类癌细胞具有抑制作用的生物活性肽的新来源的候选资格。
实际应用
细胞凋亡是维持多细胞生物完整性的重要生物学机制，细胞凋亡失败可能导致人类恶性肿瘤的发生。Rapidol是世界上第二大蛋白质粉来源，有可能被用作食品成分，而不是被浪费或用于动物饲料。在这项研究中，我们确定了一个菜籽肽级分，从菜籽粕制备的生物活性肽的来源与抗癌特性对人类癌细胞通过诱导凋亡。此外，我们以前的研究表明，菜籽肽水解物可用作具有抗氧化特性的人类食品添加剂。因此，未充分利用的副产品如脱脂菜籽粕可作为新型治疗剂用于抗癌和开发新的功能食品。
One rapeseed peptide fraction (RSP2) was prepared from rapeseed meal by enzyme digestion and separation on Sephadex gel-filtration column. The anticancer effects of RSP2 on human cervical HeLa cell lines were evaluated. Exposure of RSP2 caused a significant decrease in viability of HeLa cells in a dose-dependent manner and typical morphological changes and apoptosis bleb phenomenon in RSP2-treated HeLa cells were observed. DNA fragmentation assay and single-cell gel electrophoresis showed that RSP2 could induce apoptosis in HeLa cells at all concentrations tested in a dose-dependent manner. Together with our recently published data that RSP2 could decrease the percentage of HeLa cells in the G0/G1phase from 55% to 43%, and increase the ratio of apoptotic cells from 2.9 to 40%, results of this study highlighted the candidacy of RSP2 as a novel source of biologically active peptides with an inhibiting effect on human cancer cells.
PRACTICAL APPLICATIONS
Apoptosis is an important biological mechanism to maintain the integrity of multicellular organisms and the failure of apoptosis could lead to the development of human malignancies. Rapeseed is the world's second leading source of protein meal and could potentially be used as a food ingredient instead of being wasted or being used for animal feed. In this study, we identified one rapeseed peptide fraction prepared from rapeseed meal as a source of bioactive peptides with anticarcinogenic properties on human cancer cell through the induction of apoptosis. In addition, our previous study showed rapeseed peptide hydrolysate may be useful as a human food addition with antioxidant properties. Therefore, an underutilized by-product such as defatted rapeseed meal could be utilized as novel therapeutic agents to fight against cancer and develop new functional foods.
影响因子：
56.9
作者：
Hong, WK;Sporn, MB
通讯作者：
Sporn, MB