Regulation of transepithelial ion transport by two different purinoceptors in the apical membrane of canine kidney (MDCK) cells
Regulation of transepithelial ion transport by two different purinoceptors in the apical membrane of canine kidney (MDCK) cells
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犬肾（MDCK）细胞顶膜中两种不同嘌呤受体对跨上皮离子转运的调节
DOI：
10.1111/j.1476-5381.1995.tb13312.x
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发表时间：
1995
影响因子：
7.3
通讯作者：
L. Galietta
中科院分区：
文献类型：
--
作者：
O. Zegarra‐Moran;G. Romeo;L. Galietta
1研究了细胞外核苷酸对Madin达比犬肾细胞（MDCK）跨上皮离子转运的影响。细胞在可渗透的支持物上生长至汇合，并使用Ussing室样微灌注系统测量短路电流（Isc）。2.心尖部ATP刺激双相Isc增加，包括第一个快速和短暂的峰，然后是更宽的峰。3碱性蓝（BB）可浓度依赖性地可逆性阻断ATP诱发的第一峰，EC 50为7.5 μm。4 P2 Y受体激动剂2-甲硫基ATP（2-MeSATP）引起单次短暂的Isc增加，该增加被BB预处理完全阻断。相反，P2 x激动剂α，β-亚甲基ATP（α，β-meATP）对Isc几乎完全无效。UTP基本上诱导了单相反应，其时间过程类似于ATP刺激的第二个峰。对于第二个峰，激动剂效力顺序为2-MeSATP ≥ ATP >> UTP，α 2-MeSATP > α，β-meATP。5与膜渗透性钙螯合剂[双邻氨基苯氧基）乙烷-N，N，N′，N′，-四乙酸，四（乙酰基甲基）酯]（BAPTA/AM）单层孵育抑制ATP诱发的第一峰。6不可水解的ATP类似物腺苷-5 ′-O-（3-硫代）-三磷酸（ATP-γ-S）引起与ATP类似的双相反应。P1受体激动剂2-氯腺苷和CGS-21680几乎不能诱导Isc增加。这些结果排除了参与ATP水解和P1受体激活负责Isc增加。7吲哚美辛抑制三尖杉酯碱合成可消除ATP诱发的第二个峰。8在上皮两侧用葡萄糖酸盐置换氯完全抑制ATP诱导的第二峰，但仅降低第一峰的幅度。结果提示ATP通过两种机制刺激Isc增加。第一种是由P2 Y受体和细胞内钙增加介导的。第二种可能通过P2 U受体激活诱导前列腺素合成。
1 The effect of extracellular nucleotides on the transepithelial ion transport of Madin Darby canine kidney cells (MDCK) was investigated. Cells were grown up to confluency on permeable supports and the short circuit current (Isc) was measured with an Ussing chamber‐like mini‐perfusion system. 2 Apical ATP stimulated a biphasic Isc increase consisting of a first rapid and transient peak followed by a broader one. 3 The first peak evoked by ATP was reversibly blocked by basilen blue (BB) in a concentration‐dependent fashion, with an EC50 of 7.5 μm. 4 The P2Y receptor agonist, 2‐methylthioATP (2‐MeSATP) caused a single transient Isc increase that was completely blocked by pretreatment with BB. On the contrary, the P2x agonist, α,β‐methylene ATP (α,β‐meATP) was almost completely ineffective on Isc. UTP essentially induced a monophasic response the time‐course of which resembled that of the second peak stimulated by ATP. The agonist potency order was 2‐MeSATP ≥ ATP >> UTP, α 2‐MeSATP > α,β‐meATP for the second peak. 5 Monolayer incubation with the membrane permeable calcium chelator [bis‐o‐aminophenoxy)‐ethane‐N,N,N′,N′,‐tetraacetic acid, tetra(acetoximethyl)‐ester] (BAPTA/AM) inhibited the ATP‐evoked first peak. 6 The non‐hydrolyzable ATP analogue, adenosine‐5′‐O‐(3‐thio)‐trisphosphate (ATP‐γ‐S) elicited a biphasic response similar to that of ATP. The P1 receptor agonist, 2‐chloroadenosine and CGS‐21680, were almost unable to induce an Isc increase. These results rule out the involvement of ATP hydrolysis and P1 receptor activation as responsible for Isc increase. 7 Inhibition of prostaglandins synthesis by indomethacin abolished the second ATP‐evoked peak. 8 Chloride replacement with gluconate on both sides of the epithelium completely inhibited the second peak induced by ATP but only reduced the amplitude of the first spike. 9 The results suggest that ATP stimulates Isc increase by two mechanisms. The first one is mediated by a P2Y receptor and by intracellular calcium increase. The second induces prostaglandin synthesis probably through a P2U receptor activation.
使用单克隆抗体分离皮质集合管细胞：AVP 诱导 PGE 释放。
DOI：
10.1152/ajpcell.1983.244.3.c211
发表时间：
1983
期刊：
The American journal of physiology
影响因子：
--
作者：
Garcia-Perez,A;Smith,WL
通讯作者：
Smith,WL
Madin-Darby 犬肾细胞中 α 1-肾上腺素能受体介导的磷酸肌醇水解和前列腺素 E2 形成。
DOI：
--
发表时间：
1987
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Slivka,SR;Insel,PA
通讯作者：
Insel,PA
佛波酯和新霉素在 Madin-Darby 犬肾细胞中解离缓激肽受体介导的花生四烯酸释放和多磷酸肌醇水解。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Slivka,SR;Insel,PA
通讯作者：
Insel,PA