Effect of surface charge on the colloidal stability and in vitro uptake of carboxymethyl dextran-coated iron oxide nanoparticles.
Effect of surface charge on the colloidal stability and in vitro uptake of carboxymethyl dextran-coated iron oxide nanoparticles.
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DOI：
10.1007/s11051-013-1874-0
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发表时间：
2013-08-01
影响因子：
2.5
通讯作者：
Rinaldi, Carlos
中科院分区：
文献类型：
--
作者：
Ayala, Vanessa;Herrera, Adriana P.;Latorre-Esteves, Magda;Torres-Lugo, Madeline;Rinaldi, Carlos
纳米颗粒的物理化学性质（例如表面电荷）被认为在细胞摄取和颗粒与细胞相互作用中发挥着重要作用。为了系统地评估表面电荷对氧化铁纳米颗粒吸收的作用，我们制备了具有不同取代度（每条链 38 至 5 个基团）的羧甲基取代葡聚糖，并使用碳二亚胺化学将其与粒径分布在 33-45 nm 范围内的胺硅烷涂覆的氧化铁纳米颗粒进行反应。通过 Zeta 电位测量确定，羧甲基取代的葡聚糖涂覆的纳米颗粒的表面电荷范围为 -50 至 5 mV，并且取决于葡聚糖链中并入的羧甲基基团的数量。纳米颗粒与 CaCo-2 人结肠癌细胞一起孵育。通过共焦激光扫描显微镜观察纳米颗粒与细胞的相互作用，并通过使用电感耦合等离子体质谱法的元素分析来量化吸收。使用液相、网格蛋白介导和小窝介导的内吞作用的药理学抑制剂推断内化机制。结果显示负电荷较多的纳米粒子的吸收增加。内化模式表明，带负电最多的粒子的吸收是通过非特异性相互作用发生的。
Nanoparticle physicochemical properties such as surface charge are considered to play an important role in cellular uptake and particle–cell interactions. In order to systematically evaluate the role of surface charge on the uptake of iron oxide nanoparticles, we prepared carboxymethyl-substituted dextrans with different degrees of substitution, ranging from 38 to 5 groups per chain, and reacted them using carbodiimide chemistry with amine–silane-coated iron oxide nanoparticles with narrow size distributions in the range of 33–45 nm. Surface charge of carboxymethyl-substituted dextran-coated nano-particles ranged from −50 to 5 mV as determined by zeta potential measurements, and was dependent on the number of carboxymethyl groups incorporated in the dextran chains. Nanoparticles were incubated with CaCo-2 human colon cancer cells. Nanoparticle–cell interactions were observed by confocal laser scanning microscopy and uptake was quantified by elemental analysis using inductively coupled plasma mass spectroscopy. Mechanisms of internalization were inferred using pharmacological inhibitors for fluid-phase, clathrin-mediated, and caveola-mediated endocytosis. Results showed increased uptake for nanoparticles with greater negative charge. Internalization patterns suggest that uptake of the most negatively charged particles occurs via non-specific interactions.
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DOI：
10.1016/j.ejpb.2012.03.009
发表时间：
2012-06-01
影响因子：
4.9
作者：
Alhareth, Khairallah;Vauthier, Christine;Moussa, Fathi
通讯作者：
Moussa, Fathi
影响因子：
--
作者：
Chen, Jyh-Ping;Yang, Pei-Chin;Lu, Yu-Jen
通讯作者：
Lu, Yu-Jen
影响因子：
--
作者：
Creixell, Mar;Herrera, Adriana P.;Rinaldi, Carlos
通讯作者：
Rinaldi, Carlos
影响因子：
7.4
作者：
EYLER, RW;KLUG, ED;DIEPHUIS, F
通讯作者：
DIEPHUIS, F
影响因子：
5.8
作者：
Gautier, J.;Munnier, E.;Chourpa, I.
通讯作者：
Chourpa, I.