Genome-wide analysis of alternative splicing during dendritic cell response to a bacterial challenge.
Genome-wide analysis of alternative splicing during dendritic cell response to a bacterial challenge.
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DOI：
10.1371/journal.pone.0061975
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Moita L
中科院分区：
文献类型：
--
作者：
Rodrigues R;Grosso AR;Moita L
免疫系统依赖其组成部分的可塑性来对频繁的环境挑战做出适当的反应。树突状细胞(DC)是先天免疫的重要启动者，协调较晚的、更具特异性的获得性免疫。转录程序中多样性的产生是有效免疫反应的核心。选择性剪接被广泛认为是转录和蛋白质组复杂性的关键因素，但它在免疫细胞中的作用很少被系统地讨论。在这里，我们使用剪接敏感阵列来评估人类DC在细菌挑战下的全基因组基因和外显子水平的表达谱。我们发现广泛存在的选择性剪接事件和剪接因子转录签名由大肠杆菌对人类DC的挑战而诱导。选择性剪接与转录调控协同作用，但这两种基因调控机制主要影响不同的功能基因组。选择性剪接可能在DC免疫生物学中发挥重要作用，因为它影响与DC发育、内吞、抗原呈递和细胞周期停滞有关的基因。
The immune system relies on the plasticity of its components to produce appropriate responses to frequent environmental challenges. Dendritic cells (DCs) are critical initiators of innate immunity and orchestrate the later and more specific adaptive immunity. The generation of diversity in transcriptional programs is central for effective immune responses. Alternative splicing is widely considered a key generator of transcriptional and proteomic complexity, but its role has been rarely addressed systematically in immune cells. Here we used splicing-sensitive arrays to assess genome-wide gene- and exon-level expression profiles in human DCs in response to a bacterial challenge. We find widespread alternative splicing events and splicing factor transcriptional signatures induced by an E. coli challenge to human DCs. Alternative splicing acts in concert with transcriptional modulation, but these two mechanisms of gene regulation affect primarily distinct functional gene groups. Alternative splicing is likely to have an important role in DC immunobiology because it affects genes known to be involved in DC development, endocytosis, antigen presentation and cell cycle arrest.
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影响因子：
64.5
作者：
GYURIS, J;GOLEMIS, E;BRENT, R
通讯作者：
BRENT, R
影响因子：
5.4
作者：
Heufler, C;Koch, F;Schuler, G
通讯作者：
Schuler, G
DOI：
10.1016/j.bbrc.2010.09.133
发表时间：
2010-11-26
影响因子：
3.1
作者：
Gao Jialin;Gu Xuefan;Zhang Huiwen
通讯作者：
Zhang Huiwen
影响因子：
56.9
作者：
Golub, TR;Slonim, DK;Lander, ES
通讯作者：
Lander, ES
影响因子：
20.3
作者：
Ahn, JH;Lee, Y;Bae, YS
通讯作者：
Bae, YS