Limited predictive value of blastomere angle of division in trophectoderm and inner cell mass specification.
Limited predictive value of blastomere angle of division in trophectoderm and inner cell mass specification.
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DOI：
10.1242/dev.103267
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发表时间：
2014-06
期刊：
影响因子：
--
通讯作者：
Srinivas S
中科院分区：
文献类型：
--
作者：
Watanabe T;Biggins JS;Tannan NB;Srinivas S
滋养外胚层（TE）和多能性内细胞团（ICM）的形成是哺乳动物胚胎发生过程中最早的事件之一。据信，在8-和16-细胞期胚胎中极化卵裂球的分裂方向决定了子细胞的命运，这是基于不对称分布的谱系决定因素是如何分离的。为了研究未受干扰的胚胎分裂角度和随后的命运之间的关系，我们构建了小鼠胚胎从桑椹胚到早期囊胚阶段发育的细胞分辨率数字表示，基于4D共聚焦图像体积。我们发现，在16细胞阶段，由于细胞分裂，最初产生的内部细胞很少，但由于细胞运动，内部细胞的数量增加。与预期相反，在16细胞阶段的外部细胞代表了异质群体，其中一些注定只对TE起作用，而另一些则能够对TE和ICM起作用。我们的数据支持这样的观点，即除了分裂角度以外的因素，如卵裂球的位置，在TE和ICM的特化中起着重要作用。
The formation of trophectoderm (TE) and pluripotent inner cell mass (ICM) is one of the earliest events during mammalian embryogenesis. It is believed that the orientation of division of polarised blastomeres in the 8- and 16-cell stage embryo determines the fate of daughter cells, based on how asymmetrically distributed lineage determinants are segregated. To investigate the relationship between angle of division and subsequent fate in unperturbed embryos, we constructed cellular resolution digital representations of the development of mouse embryos from the morula to early blastocyst stage, based on 4D confocal image volumes. We find that at the 16-cell stage, very few inside cells are initially produced as a result of cell division, but that the number increases due to cell movement. Contrary to expectations, outside cells at the 16-cell stage represent a heterogeneous population, with some fated to contributing exclusively to the TE and others capable of contributing to both the TE and ICM. Our data support the view that factors other than the angle of division, such as the position of a blastomere, play a major role in the specification of TE and ICM.
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影响因子：
5.4
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