Gene regulation in parthenocarpic tomato fruit.
Gene regulation in parthenocarpic tomato fruit.
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DOI：
10.1093/jxb/erp227
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发表时间：
2009
影响因子：
6.9
通讯作者：
Dandekar AM
中科院分区：
文献类型：
--
作者：
Martinelli F;Uratsu SL;Reagan RL;Chen Y;Tricoli D;Fiehn O;Rocke DM;Gasser CS;Dandekar AM
单性结实对于许多商业种植的水果来说是潜在的期望性状，如果可以避免结构、风味或营养的不期望的变化。通过来自拟南芥（Arabidopsis thaliana）的DefH 9或INNER NO OUTER（INO）启动子驱动的生长素合成（iaaM）或响应性（rolB）基因的调控获得单性结实转基因番茄植物（cv MicroTom）。在一个断路器阶段的水果进行了分析，在转录组学和代谢组学水平，使用微阵列，实时逆转录-聚合酶链反应（RT-PCR）和飞马III TOF（飞行时间）质谱仪。虽然观察到完全成熟的果实的形状的差异，没有明确的相关性，可以在种子的数量，转基因和果实大小。这两种启动子表达生长素合成或反应基因产生无籽单性结实果实。83%的基因测量显示没有显着差异，由于单性结实的表达。具有显著变异（P <0.05）的剩余17%（1748个基因）通过基于BLAST将预测功能（当已知时）分配给TAIR数据库来研究。其中有几个基因属于细胞壁，激素代谢和反应（特别是生长素），糖和脂质的代谢。在转基因单性结实果实中观察到脂转运蛋白的上调和几个吲哚-3-乙酸（IAA）和乙烯相关基因的差异表达。尽管几种脂肪酸、氨基酸和其他代谢物存在差异，但基本代谢特征保持不变。这项工作表明，单性结实与胚珠特异性改变生长素的合成或响应驱动的INO启动子可以有效地应用这种变化是商业上可取的。
Parthenocarpy is potentially a desirable trait for many commercially grown fruits if undesirable changes to structure, flavour, or nutrition can be avoided. Parthenocarpic transgenic tomato plants (cv MicroTom) were obtained by the regulation of genes for auxin synthesis (iaaM) or responsiveness (rolB) driven by DefH9 or the INNER NO OUTER (INO) promoter from Arabidopsis thaliana. Fruits at a breaker stage were analysed at a transcriptomic and metabolomic level using microarrays, real-time reverse transcription-polymerase chain reaction (RT-PCR) and a Pegasus III TOF (time of flight) mass spectrometer. Although differences were observed in the shape of fully ripe fruits, no clear correlation could be made between the number of seeds, transgene, and fruit size. Expression of auxin synthesis or responsiveness genes by both of these promoters produced seedless parthenocarpic fruits. Eighty-three percent of the genes measured showed no significant differences in expression due to parthenocarpy. The remaining 17% with significant variation (P <0.05) (1748 genes) were studied by assigning a predicted function (when known) based on BLAST to the TAIR database. Among them several genes belong to cell wall, hormone metabolism and response (auxin in particular), and metabolism of sugars and lipids. Up-regulation of lipid transfer proteins and differential expression of several indole-3-acetic acid (IAA)- and ethylene-associated genes were observed in transgenic parthenocarpic fruits. Despite differences in several fatty acids, amino acids, and other metabolites, the fundamental metabolic profile remains unchanged. This work showed that parthenocarpy with ovule-specific alteration of auxin synthesis or response driven by the INO promoter could be effectively applied where such changes are commercially desirable.
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影响因子：
7.2
作者：
Meister, RJ;Williams, LA;Gasser, CS
通讯作者：
Gasser, CS
影响因子：
3.5
作者：
Pandolfini T;Rotino GL;Camerini S;Defez R;Spena A
通讯作者：
Spena A
影响因子：
7.4
作者：
Goetz, Marc;Hooper, Lauren C.;Koltunow, Anna M.
通讯作者：
Koltunow, Anna M.
影响因子：
46.9
作者：
Fiehn, O;Kopka, J;Willmitzer, L
通讯作者：
Willmitzer, L
DOI：
10.1080/14620316.2003.11511701
发表时间：
2003-11-01
影响因子：
1.9
作者：
Janoudi, A;Flore, JA
通讯作者：
Flore, JA