Determining the repertoire of immunodominant proteins via whole-genome amplification of intracellular pathogens.
Determining the repertoire of immunodominant proteins via whole-genome amplification of intracellular pathogens.
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DOI：
10.1371/journal.pone.0036456
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Barbet AF
中科院分区：
文献类型：
--
作者：
Dark MJ;Lundgren AM;Barbet AF
培养许多专性胞内细菌是困难的，甚至是不可能的。然而，这些生物具有许多适应能力，允许感染持久性和免疫系统逃避，使它们成为最有趣的研究对象之一。基因组测序、焦磷酸测序和Phi29扩增方面的最新进展使无需培养即可检查细胞内细菌的全基因组序列成为可能。我们将这两种技术应用于细胞内病原体边缘无浆体和人类病原体吞噬无浆体的模型，以检测phi29扩增的能力，以确定允许免疫系统逃避和在宿主中长期存在的基因序列。与传统的焦磷酸测序相比，phi29介导的基因组扩增具有相似的基因组覆盖率，在覆盖率上没有额外的差距。此外，从phi29扩增的基因组中检测到并提取了来自两个边缘农杆菌菌株的所有msp2功能假基因，这突显了它在确定参与免疫逃避的全部基因方面的有效性。
Culturing many obligate intracellular bacteria is difficult or impossible. However, these organisms have numerous adaptations allowing for infection persistence and immune system evasion, making them some of the most interesting to study. Recent advancements in genome sequencing, pyrosequencing and Phi29 amplification, have allowed for examination of whole-genome sequences of intracellular bacteria without culture. We have applied both techniques to the model obligate intracellular pathogen Anaplasma marginale and the human pathogen Anaplasma phagocytophilum, in order to examine the ability of phi29 amplification to determine the sequence of genes allowing for immune system evasion and long-term persistence in the host. When compared to traditional pyrosequencing, phi29-mediated genome amplification had similar genome coverage, with no additional gaps in coverage. Additionally, all msp2 functional pseudogenes from two strains of A. marginale were detected and extracted from the phi29-amplified genomes, highlighting its utility in determining the full complement of genes involved in immune evasion.
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影响因子：
4.5
作者：
Dunning Hotopp JC;Lin M;Madupu R;Crabtree J;Angiuoli SV;Eisen JA;Seshadri R;Ren Q;Wu M;Utterback TR;Smith S;Lewis M;Khouri H;Zhang C;Niu H;Lin Q;Ohashi N;Zhi N;Nelson W;Brinkac LM;Dodson RJ;Rosovitz MJ;Sundaram J;Daugherty SC;Davidsen T;Durkin AS;Gwinn M;Haft DH;Selengut JD;Sullivan SA;Zafar N;Zhou L;Benahmed F;Forberger H;Halpin R;Mulligan S;Robinson J;White O;Rikihisa Y;Tettelin H
通讯作者：
Tettelin H
影响因子：
3.5
作者：
Felsheim RF;Herron MJ;Nelson CM;Burkhardt NY;Barbet AF;Kurtti TJ;Munderloh UG
通讯作者：
Munderloh UG
影响因子：
3.6
作者：
Futse, JE;Brayton, KA;Palmer, GH
通讯作者：
Palmer, GH
DOI：
10.1073/pnas.0710333105
发表时间：
2008-02-12
影响因子：
11.1
作者：
Futse, James E.;Brayton, Kelly A.;Palmer, Guy H.
通讯作者：
Palmer, Guy H.
影响因子：
7
作者：
Smith, Douglas R.;Quinlan, Aaron R.;Richardson, Paul M.
通讯作者：
Richardson, Paul M.