Efficient delivery of antisense oligodeoxyribonucleotide g3139 by human serum albumin-coated liposomes.
Efficient delivery of antisense oligodeoxyribonucleotide g3139 by human serum albumin-coated liposomes.
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DOI：
10.1021/mp900150g
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发表时间：
2009-11
影响因子：
4.9
通讯作者：
Lee RJ
中科院分区：
文献类型：
--
作者：
Weecharangsan W;Yu B;Zheng Y;Liu S;Pang JX;Lee LJ;Marcucci G;Lee RJ
Human serum albumin (HSA)-coated liposomal formulations were synthesized and evaluated for the delivery of antisense oligodeoxyribonucleotide (ODN) G3139 in KB human oral carcinoma cells. Liposomes composed of dimethyldioctadecyl ammonium bromide/egg phosphatidylcholine/α-tocopheryl polyethylene glycol 1000 succinate (58:40:2 molar ratio) complexed with G3139 and coated with HSA were investigated for Bcl-2 down regulating activity. Cellular uptake of HSA-coated liposome-ODN complexes was more efficient than the uncoated liposome-ODN complexes. Treatment of the cells with HSA-coated liposome-ODN complexes resulted in efficient Bcl-2 mRNA down regulation that was approximately 3-fold greater than with uncoated liposomes (p<0.05) and 6-fold greater than with free ODN. The transfection efficiency of liposome-ODN complexes coated with HSA was dependent on the concentration of HSA used and on the contents of α-helix and β-strand in HSA. HSA-coated liposomes are effective delivery vehicles for antisense ODN.
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