α-Interferon-induced transcription of HLA and metallothionein genes containing homologous upstream sequences
α-Interferon-induced transcription of HLA and metallothionein genes containing homologous upstream sequences
复制标题
α-干扰素诱导含有同源上游序列的 HLA 和金属硫蛋白基因的转录
作者：
R. Friedman;G. Stark
最近已分离出与干扰素 (IFN) 诱导的组织相容性位点抗原 (HLA)、金属硫蛋白-II (MT2)、2',5'-寡腺苷酸合成酶和大约八种其他未知序列蛋白质相对应的信使 RNA 的互补 DNA1-5，并且已证明干扰素对八种 mRNA 中的几种 mRNA 的转录调节作用3,4，在短短 5 分钟内即可明显增加。我们现在表明，IFN-α 治疗可使人 T98G 神经母细胞瘤细胞中 MT2 和 HLA I 类基因的转录增加三到五倍。此外，对 MT2A 基因、两个 HLA 基因和一个 HLA II 类基因上游区域的比较揭示了约 30 个碱基对 (bp) 的同源序列，该序列可能参与调节干扰素诱导基因的转录。人 MT2A mRNA 的转录是由糖皮质激素或金属离子 6,7 诱导的，并且调节元件已通过启动子融合实验 8 进行了定位。我们现在表明，MT2A 的转录速率与干扰素或地塞米松治疗时相同，但 mRNA 在地塞米松治疗下积累得更快，表明转录后事件在后一种情况下很重要。
Complementary DNAs corresponding to the Interferon (IFN)-induced messenger RNAs for histocompatibility locus antigens (HLA), metallothionein-II (MT2), 2′,5′-oligoadenylate synthetase and about eight other proteins of unknown sequence have been isolated recently1–5, and by interferon regulation of transcription has been demonstrated for several of the eight mRNAs3,4, with a significant increase apparent in as little as 5 min3. We now show that IFN-α treatment results in a three- to fivefold increase in the transcription of MT2 and HLA class I genes in human T98G neuroblastoma cells. Furthermore, comparison of regions upstream of the MT2A gene, two HLA genes and one HLA class II gene reveals a homologous sequence of ∼30 base pairs (bp) which may be involved in regulating transcription of interf eron-induced genes. Transcription of the mRNA for human MT2A is induced by glucocorticoids or metal ions6,7 and the regulatory elements have been mapped by promoter-fusion experiments8. We now show that the rate of transcription of MT2A is the same on treatment with interferon or dexamethasone, but that the mRNA accumulates much faster with dexamethasone, indicating that post-transcriptional events are important in the latter case.
糖皮质激素介导的 α1-酸性糖蛋白诱导：激素调节 RNA 加工的证据。
DOI：
10.1073/pnas.81.14.4241
发表时间：
1984
影响因子：
11.1
作者：
Vannice,JL;Taylor,JM;Ringold,GM
通讯作者：
Ringold,GM
转染小鼠细胞中缺少 5 末端区域的人类 HLA 基因片段的转录本。
DOI：
10.1073/pnas.81.3.649
发表时间：
1984
影响因子：
11.1
作者：
Yoshie,O;Schmidt,H;Lengyel,P;Reddy,ES;Morgan,WR;Weissman,SM
通讯作者：
Weissman,SM
DOI：
10.1073/pnas.81.21.6733
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
LARNER, AC;JONAK, G;DARNELL, JE
通讯作者：
DARNELL, JE