Bacterial and fungal microflora in surgically removed lung cancer samples.
Bacterial and fungal microflora in surgically removed lung cancer samples.
复制标题
手术中的细菌和真菌菌群去除肺癌样品。
DOI：
10.1186/1749-8090-6-137
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发表时间：
2011-10-14
影响因子：
1.6
通讯作者：
Giamouzis G
中科院分区：
文献类型：
--
作者：
Apostolou P;Tsantsaridou A;Papasotiriou I;Toloudi M;Chatziioannou M;Giamouzis G
临床和实验数据表明，细菌和/或真菌感染的存在与不同类型癌症的发展之间存在关联，与化疗引起的白细胞减少症无关。这也被假设为肺癌的发展，但尚未描述所涉及的细菌和真菌的患病率和确切种类。确定手术提取的肺癌患者样本中是否存在细菌和真菌微生物群。在这项单中心前瞻性观察性研究中，通过手术从连续 32 名肺癌患者中提取组织样本，并使用逆转录聚合酶链反应 (RT-PCR) 来鉴定细菌和真菌菌株的存在。电泳数据的分析指出了样品和已鉴定的菌株之间的多样性。所有样品中均鉴定出支原体菌株。出现频率较高的菌株是表皮葡萄球菌、轻链球菌和芽孢杆菌菌株，其次是衣原体、念珠菌、李斯特菌和流感嗜血杆菌。在个别患者中检测到嗜肺军团菌和热带念珠菌。在肺癌患者手术提取的组织样本中可以鉴定出多种病原体，所有样本中都存在支原体菌株。这些结果表明慢性感染在肺癌发生中的病因学作用。需要证实这些观察结果并进行更多研究，以进一步确定炎症在肺癌发生中的病因作用。
Clinical and experimental data suggest an association between the presence of bacterial and/or fungal infection and the development of different types of cancer, independently of chemotherapy-induced leukopenia. This has also been postulated for the development of lung cancer, however the prevalence and the exact species of the bacteria and fungi implicated, have not yet been described. To determine the presence of bacterial and fungal microflora in surgically extracted samples of patients with lung cancer. In this single-center prospective, observational study, tissue samples were surgically extracted from 32 consecutive patients with lung cancer, and reverse-transcription polymerase chain reaction (RT-PCR) was used to identify the presence of bacteria and fungi strains. The analysis of the electrophoresis data pointed out diversity between the samples and the strains that were identified. Mycoplasma strains were identified in all samples. Strains that appeared more often were Staphylococcus epidermidis, Streptococcus mitis and Bacillus strains, followed in descending frequency by Chlamydia, Candida, Listeria, and Haemophilus influenza. In individual patients Legionella pneumophila and Candida tropicalis were detected. A diversity of pathogens could be identified in surgically extracted tissue samples of patients with lung cancer, with mycoplasma strains being present in all samples. These results point to an etiologic role for chronic infection in lung carcinogenesis. Confirmation of these observations and additional studies are needed to further characterize the etiologic role of inflammation in lung carcinogenesis.
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影响因子：
3.6
作者：
Ballaz, Santiago;Mulshine, James L
通讯作者：
Mulshine, James L
影响因子：
14.2
作者：
Dendis, M;Horváth, R;Benedík, J
通讯作者：
Benedík, J
影响因子：
7
作者：
Hsieh, Sen-Yung;Tseng, Chiao-Li;Chen, Jen-Kun
通讯作者：
Chen, Jen-Kun
影响因子：
0.6
作者：
Laroumagne, S.;Salinas-Pineda, A.;Mazieres, J.
通讯作者：
Mazieres, J.
影响因子：
2.8
作者：
Koyi, H;Brandén, E;Hillerdal, G
通讯作者：
Hillerdal, G