Increased interferon alpha receptor 2 mRNA levels is associated with renal cell carcinoma metastasis.-猫眼课题宝

DOI：

10.1186/1471-2407-7-159

发表时间：

2007-08-15

期刊：

BMC CANCER

影响因子：

3.8

通讯作者：

中科院分区：

医学2区

文献类型：

作者：

Kamai, Takao;Yanai, Yoshiaki;Arai, Kyoko;Abe, Hideyuki;Yamanishi, Tomonori;Kurimoto, Masashi;Yoshida, Ken-Ichiro

来源链接：

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1988828/?report=classic

Interferon-α (IFN-α) is one of the central agents in immunotherapy for renal cell carcinoma (RCC) and binds to the IFN-α receptor (IFNAR). We investigated the role of IFNAR in RCC. We quantified IFNAR mRNA expression in paired tumor and non-tumor samples from the surgical specimens of 103 consecutive patients with RCC using a real-time reverse transcription polymerase chain reaction (RT-PCR), and IFNAR2 protein using Western blotting. The absolute level of IFNAR1 and IFNAR2 mRNAs in tumor and non-tumor tissues did not correlate with the malignant and metastatic profiles. The relative yields of the PCR product from the tumor tissue to that from the corresponding non-tumor tissue (T/N) for the expression of IFNAR mRNAs were calculated. While the T/N ratio of IFNAR1 did not correlate with any factor, a high T/N ratio of IFNAR2 correlated with poor differentiation (P < 0.05), local invasion (P < 0.001), and metastasis (P < 0.0001). By multivariate analysis, a high T/N ratio of IFNAR2 predicted a shortened overall survival in all cases (P < 0.05) and a shorter disease-free survival in those without metastasis (M0; 68 cases, P < 0.05). Impressively, patients with a poorer response to IFN-α treatment had a higher IFNAR2 T/N ratio than those who had a good response (P < 0.05). IFNAR2c protein expression was higher in the primary tumors in patients with metastases (M1; 35 cases) compared to those without ( P < 0.0001). IFNAR2 is associated with the progression of RCC.