The bimodal lifestyle of intracellular Salmonella in epithelial cells: replication in the cytosol obscures defects in vacuolar replication.
The bimodal lifestyle of intracellular Salmonella in epithelial cells: replication in the cytosol obscures defects in vacuolar replication.
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DOI：
10.1371/journal.pone.0038732
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Steele-Mortimer O
中科院分区：
文献类型：
--
作者：
Malik-Kale P;Winfree S;Steele-Mortimer O
鼠伤寒沙门氏菌侵入上皮细胞并增殖。细胞内细菌在膜结合的液泡内复制，称为含沙门氏菌的液泡。然而，这种细菌也可以在上皮细胞的胞质溶胶中有效地复制，并且净细胞内生长是空泡和胞质溶胶复制的产物。在这里，我们已经使用半定量单细胞分析，以调查这些复制壁龛中的每一个在培养的上皮细胞的细胞内增殖的贡献。我们发现，胞质复制可以占大部分的净复制，即使它发生在不到20%的感染细胞。因此，对感染细胞群体中的净生长的测定，例如通过集落形成单位的回收，不是空泡增殖的良好指标。我们还表明，沙门氏菌III型分泌系统2，这是所需的SCV生物合成，是不需要的胞质复制。总之，这项研究说明了单细胞分析的价值时，研究细胞内病原体。
Salmonella enterica serovar Typhimurium invades and proliferates within epithelial cells. Intracellular bacteria replicate within a membrane bound vacuole known as the Salmonella containing vacuole. However, this bacterium can also replicate efficiently in the cytosol of epithelial cells and net intracellular growth is a product of both vacuolar and cytosolic replication. Here we have used semi-quantitative single-cell analyses to investigate the contribution of each of these replicative niches to intracellular proliferation in cultured epithelial cells. We show that cytosolic replication can account for the majority of net replication even though it occurs in less than 20% of infected cells. Consequently, assays for net growth in a population of infected cells, for example by recovery of colony forming units, are not good indicators of vacuolar proliferation. We also show that the Salmonella Type III Secretion System 2, which is required for SCV biogenesis, is not required for cytosolic replication. Altogether this study illustrates the value of single cell analyses when studying intracellular pathogens.
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将鼠伤寒沙门氏菌靶向含有溶酶体膜糖蛋白的囊泡，绕过带有甘露糖6-磷酸受体的室。
影响因子：
7.8
作者：
GARCIADELPORTILLO, F;FINLAY, BB
通讯作者：
FINLAY, BB
影响因子：
4
作者：
Bujny, Miriam V.;Ewels, Phil A.;Cullen, Peter J.
通讯作者：
Cullen, Peter J.
影响因子：
11.4
作者：
Kerr, Markus C.;Wang, Jack T. H.;Teasdale, Rohan D.
通讯作者：
Teasdale, Rohan D.
影响因子：
5.4
作者：
Marlovits, Thomas C.;Stebbins, C. Erec
通讯作者：
Stebbins, C. Erec
影响因子：
3.6
作者：
Knodler, LA;Vallance, BA;Steele-Mortimer, O
通讯作者：
Steele-Mortimer, O