Aging-related changes of triose phosphate isomerase in hippocampus of senescence accelerated mouse and the intervention of acupuncture
Aging-related changes of triose phosphate isomerase in hippocampus of senescence accelerated mouse and the intervention of acupuncture
复制标题
加速衰老小鼠海马磷酸丙糖异构酶的衰老相关变化及针刺干预
DOI：
10.1016/j.neulet.2013.03.002
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发表时间：
2013-05
影响因子：
2.5
通讯作者：
Yu, Jianchun
中科院分区：
文献类型：
--
作者：
Yan, Dong;Zhou, Chunlei;Han, Jingxian;Yu, Jianchun
磷酸三糖异构酶（TPI）诱导的糖代谢紊乱与阿尔茨海默病（AD）密切相关。衰老加速小鼠（Senescence accelerated mouse， SAM）常被用来作为早期认知障碍的AD模型。为了研究TPI随年龄的变化，将SAM易发8号（SAMP8）和SAM抗性1号（SAMR1）分为2个月、6个月、8个月和12个月组。为分析针刺干预效果，将SAMP8分为SAMP8对照组（Pc）、SAMP8穴位组（Pa）、SAMP8非穴位组（Pn）和SAMR1对照组（Rc）。衰老分级评分及Morris水迷宫结果显示SAMP8出现与衰老相关的学习记忆衰退，针刺可提高SAMP8的学习记忆能力。分别用比色法和Western blot法检测TPI活性和表达。与SAMR1相比，SAMP8在6、8和12个月时TPI活性显著降低。针刺干预可显著上调大鼠海马TPI活性。上述结果提示，SAMP8随着年龄增长而出现的学习记忆衰退可能与TPI活性降低有关，针刺可通过提高TPI活性改善认知功能障碍，从而纠正糖酵解代谢异常，维持大脑内环境和稳态。
Glycometabolism disorder induced by triose phosphate isomerase (TPI) is closely related to Alzheimer's disease (AD). Senescence accelerated mouse (SAM) is often employed as an AD model characteristic of early cognitive impairment. In order to investigate the variation of TPI with aging, SAM prone 8 (SAMP8) and SAM resistant 1 (SAMR1) were divided into 2-month, 6-month, 8-month and 12-month group. For the analysis of acupuncture intervention, SAMP8 were divided into SAMP8 control group (Pc), SAMP8 acupoint group (Pa), SAMP8 non-acupoint group (Pn) and SAMR1 control group (Rc). Grading score of senescence and Morris water maze results showed that SAMP8 presented aging-related deterioration of learning and memory, and that acupuncture could improve the learning and memory ability of SAMP8. TPI activity and expression were detected by colorimetric method and Western blot analysis, respectively. When compared to SAMR1, TPI activity in 6-, 8- and 12-month SAMP8 decreased significantly. However, acupuncture intervention markedly up-regulated TPI activity in hippocampus of Pa. These findings suggested that the learning and memory deterioration of SAMP8 with aging might be associated with the lower TPI activity and that acupuncture could improve the cognitive impairment by increasing TPI activity, thus correcting the abnormal glycolysis metabolism and maintaining the brain homeostasis and internal environment.
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影响因子：
14.5
作者：
Ossenkoppele, Rik;Zwan, Marissa D.;van Berckel, Bart N. M.
通讯作者：
van Berckel, Bart N. M.
影响因子：
5.3
作者：
Planel, E;Miyasaka, T;Takashima, A
通讯作者：
Takashima, A
影响因子：
4
作者：
Casadesus, Gemma;Gutierrez-Cuesta, Javier;Pallas, Merce
通讯作者：
Pallas, Merce
针灸改善 SAMP8 小鼠的认知缺陷并调节脑细胞增殖
影响因子：
2.5
作者：
Cheng, Haiyan;Yu, Jianchun;Han, Jingxian
通讯作者：
Han, Jingxian
DOI：
10.1111/j.1432-1033.1987.tb13388.x
发表时间：
1987-10-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
LAMBEIR, AM;OPPERDOES, FR;WIERENGA, RK
通讯作者：
WIERENGA, RK