Focus on biosensors: Looking through the lens of quantitative biology.
Focus on biosensors: Looking through the lens of quantitative biology.
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DOI：
10.1017/qpb.2021.10
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发表时间：
2021
影响因子：
--
通讯作者：
Jones, Alexander M.
中科院分区：
文献类型：
--
作者：
Rowe, James H.;Jones, Alexander M.
近年来，植物生物学家对量化体内分子和分子事件感兴趣，已经开始用直接感测分析物的遗传编码荧光生物传感器（GEFB）来补充报告系统。此类生物传感器可以允许在单个细胞水平和随着时间的推移进行测量。这些信息被证明是有价值的数学建模者有兴趣在硅片上表示生物现象，因为改进的测量可以指导改进的模型构建和模型参数化。合成生物学的进步加快了生物传感器的发展步伐，光谱兼容的生物传感器的同时表达现在允许量化信号网络中的多个节点。对于直接响应刺激的生物传感器，靶向特定的细胞隔室允许观察分析物在不同细胞器中的差异积累，为活性氧/钙信号传导和光合作用研究带来见解。结合改进的图像分析方法，生物传感器成像的进步可以帮助关闭实验和数学建模之间的循环。
In recent years, plant biologists interested in quantifying molecules and molecular events in vivo have started to complement reporter systems with genetically encoded fluorescent biosensors (GEFBs) that directly sense an analyte. Such biosensors can allow measurements at the level of individual cells and over time. This information is proving valuable to mathematical modellers interested in representing biological phenomena in silico, because improved measurements can guide improved model construction and model parametrisation. Advances in synthetic biology have accelerated the pace of biosensor development, and the simultaneous expression of spectrally compatible biosensors now allows quantification of multiple nodes in signalling networks. For biosensors that directly respond to stimuli, targeting to specific cellular compartments allows the observation of differential accumulation of analytes in distinct organelles, bringing insights to reactive oxygen species/calcium signalling and photosynthesis research. In conjunction with improved image analysis methods, advances in biosensor imaging can help close the loop between experimentation and mathematical modelling.
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