A map of cell type-specific auxin responses.

A map of cell type-specific auxin responses.
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DOI:
10.1038/msb.2013.40
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发表时间:
2013
影响因子:
9.9
通讯作者:
--
中科院分区:
生物学1区
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--
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在植物中,局部生长素浓度的变化可以触发一系列发育过程,因为不同的组织对相同的生长素刺激有不同的反应。然而,很少有人知道生长素是如何解释个别细胞类型。我们进行了一个转录组学分析生长素的反应在四个不同的组织拟南芥根,并证明不同的细胞类型显示能力离散的反应。大多数生长素反应基因在其诱导或抑制中表现出空间偏好。新的数据集被用来研究生长素如何影响细胞身份标记的组织特异性转录调控。此外,数据被用来与空间表达图的根绘制一个转录生长素响应梯度在顶端和基部分生组织。读出结果显示,数千个基因对生长素的相对反应与根纵轴沿着的表达之间存在很强的相关性。这个数据集和比较分析提供了一个转录组水平的空间分解的反应生长素在一个器官内,这种激素介导的发展的许多方面。
In plants, changes in local auxin concentrations can trigger a range of developmental processes as distinct tissues respond differently to the same auxin stimulus. However, little is known about how auxin is interpreted by individual cell types. We performed a transcriptomic analysis of responses to auxin within four distinct tissues of the Arabidopsis thaliana root and demonstrate that different cell types show competence for discrete responses. The majority of auxin-responsive genes displayed a spatial bias in their induction or repression. The novel data set was used to examine how auxin influences tissue-specific transcriptional regulation of cell-identity markers. Additionally, the data were used in combination with spatial expression maps of the root to plot a transcriptomic auxin-response gradient across the apical and basal meristem. The readout revealed a strong correlation for thousands of genes between the relative response to auxin and expression along the longitudinal axis of the root. This data set and comparative analysis provide a transcriptome-level spatial breakdown of the response to auxin within an organ where this hormone mediates many aspects of development.
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