Potency of gastrointestinal colonization and virulence of Candida auris in a murine endogenous candidiasis.
Potency of gastrointestinal colonization and virulence of Candida auris in a murine endogenous candidiasis.
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在鼠内源性念珠菌病中，念珠菌的胃肠道定植和毒力的效力。
DOI：
10.1371/journal.pone.0243223
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发表时间：
2020
期刊：
影响因子：
3.7
通讯作者：
Miyazaki Y
中科院分区：
文献类型：
--
作者：
Abe M;Katano H;Nagi M;Higashi Y;Sato Y;Kikuchi K;Hasegawa H;Miyazaki Y
耳念珠菌感染最近在世界范围内出现，该物种具有很强的定殖能力，并与高死亡率相关。然而，尚未报道菌株依赖性的定植能力和毒力差异。在本研究中，我们旨在阐明临床分离的耳念珠菌侵袭性菌株和非侵袭性菌株之间的差异。我们评估了在醋酸可的松免疫抑制下接种侵入性或非侵入性耳念珠菌菌株的野生 C57BL/6J 小鼠的定植、传播和存活率，并与白色念珠菌和光滑念珠菌感染进行比较。我们还评估了生物膜形成的效力。接种侵入性菌株的小鼠的粪便真菌负荷显着高于感染非侵入性菌株的小鼠。除了肠道定植外，接种侵入菌株的小鼠的肝脏和肾脏真菌负荷也显着升高。此外，组织病理学结果显示入侵菌株的传播和定植程度更大。关于生物膜形成能力，耳念珠菌的侵入菌株表现出明显更高的产生生物膜的能力。此外，接种侵入性菌株导致的体重损失比感染非侵入性菌株后明显更大。在醋酸可的松免疫抑制下，侵袭性菌株表现出比非侵袭性菌株更高的定植能力和胃肠道传播率，尽管耳念珠菌引起的死亡率低于白色念珠菌引起的死亡率。
Candida auris infections have recently emerged worldwide, and this species is highly capable of colonization and is associated with high levels of mortality. However, strain-dependent differences in colonization capabilities and virulence have not yet been reported. In the present study, we aimed to clarify the differences between clinically isolated invasive and non-invasive strains of C. auris. We evaluated colonization, dissemination, and survival rates in wild C57BL/6J mice inoculated with invasive or non-invasive strains of C. auris under cortisone acetate immunosuppression, comparing with those of Candida albicans and Candida glabrata infections. We also evaluated the potency of biofilm formation. Stool fungal burdens were significantly higher in mice inoculated with the invasive strains than in those infected with the non-invasive strain. Along with intestinal colonization, liver and kidney fungal burdens were also significantly higher in mice inoculated with the invasive strains. In addition, histopathological findings revealed greater dissemination and colonization of the invasive strains. Regarding biofilm-forming capability, the invasive strain of C. auris exhibited a significantly higher capacity of producing biofilms. Moreover, inoculation with the invasive strains resulted in significantly greater loss of body weight than that noted following infection with the non-invasive strain. Invasive strains showed higher colonization capability and rates of dissemination from gastrointestinal tracts under cortisone acetate immunosuppression than non-invasive strains, although the mortality rates caused by C. auris were lower than those caused by C. albicans.
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影响因子：
16.6
作者：
Munoz, Jose F.;Gade, Lalitha;Cuomo, Christina A.
通讯作者：
Cuomo, Christina A.
影响因子：
11.8
作者：
Nucci, M;Anaissie, E
通讯作者：
Anaissie, E
DOI：
10.1016/j.cmi.2015.09.018
发表时间：
2016-01
期刊：
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
影响因子：
--
作者：
Rajendran R;Sherry L;Nile CJ;Sherriff A;Johnson EM;Hanson MF;Williams C;Munro CA;Jones BJ;Ramage G
通讯作者：
Ramage G
影响因子：
29.4
作者：
Chen, Xinhua;Katchar, Kianoosh;Kelly, Ciaran P.
通讯作者：
Kelly, Ciaran P.
DOI：
10.1007/s10096-013-2027-1
发表时间：
2014-06-01
影响因子：
4.5
作者：
Chowdhary, A.;Kumar, V. Anil;Meis, J. F.
通讯作者：
Meis, J. F.