Coupling of "malic" enzyme and NADPH:NAD transhydrogenase in the energetics of Hymenolepis diminuta (Cestoda).

Coupling of "malic" enzyme and NADPH:NAD transhydrogenase in the energetics of Hymenolepis diminuta (Cestoda).
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Hymenolepis diminuta (Cestoda) 能量学中“苹果酸”酶与 NADPH:NAD 转氢酶的偶联。

DOI:
10.1016/0305-0491(84)90306-7
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发表时间:
1984
期刊:
Comparative biochemistry and physiology. B, Comparative biochemistry
影响因子:
--
通讯作者:
Fioravanti,CF
Fioravanti,CF
中科院分区:
--
文献类型:
--
作者:
McKelvey,JR;Fioravanti,CF

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乙酰吡啶NADP取代NADP促进需用Mn2+离子的膜膜绦虫线粒体“苹果酸”酶。当NAD或乙酰吡啶NAD作为辅酶时,被破坏的线粒体显示出低水平的草酰乙酸形成苹果酸脱氢酶活性。H. diminuta线粒体对乙酰吡啶NAD的显著苹果酸依赖性还原需要Mn2+离子和NADP,从而表明“苹果酸”酶和NADPH: NAD转氢酶的串联作用。线粒体制剂与草酰乙酸的孵育导致非酶脱羧反应。通过线粒体还原吡啶核苷酸氧化酶活性的极谱评估,证明了苹果酸氧化与苹果酸酶和转氢酶的电子传递耦合。
Acetylpyridine NADP replaced NADP in promoting the Mn2+ ion-requiring mitochondrial" malic" enzyme of Hymenolepis diminuta. Disrupted mitochondria displayed low levels of an apparent oxaloacetate-forming malate dehydrogenase activity when NAD or acetylpyridine NAD served as the coenzyme. Significant malate-dependent reduction of acetylpyridine NAD by H. diminuta mitochondria required Mn2+ ion and NADP, thereby indicating the tandem operation of" malic" enzyme and NADPH: NAD transhydrogenase. Incubation of mitochondrial preparations with oxaloacetate resulted in a non-enzymatic decarboxylation reaction. Coupling of malate oxidation with electron transport via the" malic" enzyme and transhydrogenase was demonstrated by polarographic assessment of mitochondrial reduced pyridine nucleotide oxidase activity.
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DOI: --
发表时间: 1972
期刊: Comparative biochemistry and physiology. B, Comparative biochemistry
影响因子: --
作者:
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成年 Hymenolepis diminuta (Cestoda) 的线粒体 NADH 氧化酶活性。
DOI: 10.1016/0305-0491(82)90511-9
发表时间: 1982
期刊: Comparative biochemistry and physiology. B, Comparative biochemistry
影响因子: --
作者:
Fioravanti,CF
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