Characterization of wax valving and μPIV analysis of microscale flow in paper-fluidic devices for improved modeling and design.
Characterization of wax valving and μPIV analysis of microscale flow in paper-fluidic devices for improved modeling and design.
复制标题
纸流体装置中微尺度流动的蜡阀特性和μPIV分析，用于改进建模和设计。
DOI：
10.1039/d2lc00297c
复制
发表时间：
2022-07-12
期刊：
影响因子：
6.1
通讯作者：
Linnes, Jacqueline C.
中科院分区：
文献类型：
--
作者：
Newsham, Emilie I.;Phillips, Elizabeth A.;Ma, Hui;Chang, Megan M.;Wereley, Steven T.;Linnes, Jacqueline C.
由于其低成本，易于使用和目标分子的无设备检测，纸流体装置是一种流行的即时诊断平台。然而，由于缺乏敏感性和无法结合更复杂的过程，例如核酸扩增或酶促信号增强，它们受到限制。为了解决这些限制，以前在纸流体装置中实施了各种阀门来控制流体阻塞和释放。然而，由于描述在分析中驱动生物物理反应的微尺度流动的有限实验数据，将阀门整合到新设备中是一个高度迭代的、耗时的过程。在本文中，我们测试并模拟了不同几何形状的热致动阀，以研究如何在精确控制致动时间、流量和流型的LFIA中更容易地实现它们。我们证明单独的体积流量测量不能估计高度可变的微尺度特性和对LFIA信号发展的影响。为了进一步量化纸流体装置的微流体特性，微颗粒图像测速法用于量化荧光纳米颗粒通过膜的流动，并显示出与体流不同的特性，这可能解释了LFIA信号产生的额外可变性。总之，我们证明了纸张流体装置的更强大的特性可以允许对参数进行微调，以实现多步骤分析的精确自动化，并为更有效的设计提供分析模型。
Paper-fluidic devices are a popular platform for point-of-care diagnostics due to their low cost, ease of use, and equipment-free detection of target molecules. They are limited, however, by their lack of sensitivity and inability to incorporate more complex processes, such as nucleic acid amplification or enzymatic signal enhancement. To address these limitations, various valves have previously been implemented in paper-fluidic devices to control fluid obstruction and release. However, incorporation of valves into new devices is a highly iterative, time-intensive process due to limited experimental data describing the microscale flow that drives the biophysical reactions in the assay. In this paper, we tested and modeled different geometries of thermally actuated valves to investigate how they can be more easily implemented in an LFIA with precise control of actuation time, flow rate, and flow pattern. We demonstrate that bulk flow measurements alone cannot estimate the highly variable microscale properties and effects on LFIA signal development. To further quantify the microfluidic properties of paper-fluidic devices, micro-particle image velocimetry was used to quantify fluorescent nanoparticle flow through the membranes and demonstrated divergent properties from bulk flow that may explain additional variability in LFIA signal generation. Altogether, we demonstrate that a more robust characterization of paper-fluidic devices can permit fine-tuning of parameters for precise automation of multi-step assays and inform analytical models for more efficient design.
登录
查看更多内容
DOI：
10.1007/978-1-59745-240-3_1
发表时间：
2008-10-31
期刊：
Lateral Flow Immunoassay
影响因子：
--
作者：
O’Farrell B
通讯作者：
O’Farrell B
影响因子：
4.7
作者：
Mehmani, Yashar;Tchelepi, Hamdi A.
通讯作者：
Tchelepi, Hamdi A.
影响因子：
1.2
作者：
Crannell, Zachary A.;Rohrman, Brittany;Richards-Kortum, Rebecca
通讯作者：
Richards-Kortum, Rebecca
影响因子：
7.4
作者：
Crannell, Zachary;Castellanos-Gonzalez, Alejandro;Richards-Kortum, Rebecca
通讯作者：
Richards-Kortum, Rebecca
影响因子：
4.6
作者：
Jauset-Rubio M;Svobodová M;Mairal T;McNeil C;Keegan N;Saeed A;Abbas MN;El-Shahawi MS;Bashammakh AS;Alyoubi AO;O Sullivan CK
通讯作者：
O Sullivan CK