A 104 kDa Aedes aegypti aminopeptidase N is a putative receptor for the Cry11Aa toxin from Bacillus thuringiensis subsp. israelensis.
A 104 kDa Aedes aegypti aminopeptidase N is a putative receptor for the Cry11Aa toxin from Bacillus thuringiensis subsp. israelensis.
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DOI：
10.1016/j.ibmb.2013.09.007
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发表时间：
2013-12
影响因子：
3.8
通讯作者：
Gill, Sarjeet S.
中科院分区：
文献类型：
--
作者：
Chen, Jianwu;Likitvivatanavong, Supaporn;Aimanova, Karlygash G.;Gill, Sarjeet S.
苏云金芽孢杆菌（Bacillus thuringiensis subsp.）Israelensis是一种在世界范围内用于控制埃及伊蚊幼虫的细菌菌株，其以29.8 nM的表观Kd结合中肠刷状缘膜囊泡（BBMV）。以前，通过使用生物素化的Cry 11 Aa毒素的下拉测定（pull-down assay），将命名为AaeAPN 2的氨肽酶N（APN）鉴定为推定的Cry 11 Aa毒素结合蛋白（Insect Biochem Mol Biol.，39：688-696）。在这里，我们发现这种蛋白定位于近端和远端地区的幼虫盲肠上皮细胞的顶膜。AaeAPN 2蛋白以8.6 nM的高亲和力与Cry 11 Aa结合。克隆了AaeAPN 2的全长和片段，并在大肠杆菌中表达。进一步的竞争性分析表明，Cry 11 Aa与BBMV的结合被全长AaeAPN 2和AaeAPN 2b和AaeAPN 2 e的片段有效地竞争。在对Ae.埃及伊蚊幼虫，全长和部分片段（AaeAPN 2b）的AaeAPN 2增强Cry 11 Aa幼虫死亡率的存在。综上所述，我们得出结论，AaeAPN 2是一种结合蛋白，并在Cry 11 Aa毒性中发挥作用。
The Cry11Aa protein produced in Bacillus thuringiensis subsp. israelensis, a bacterial strain used worldwide for the control of Aedes aegypti larvae, binds midgut brush border membrane vesicles (BBMV) with an apparent Kd of 29.8 nM. Previously an aminopeptidase N (APN), named AaeAPN2, was identified as a putative Cry11Aa toxin binding protein by pull-down assays using biotinylated Cry11Aa toxin ( Insect Biochem Mol Biol., 39: 688–696). Here we show this protein localizes to the apical membrane of epithelial cells in proximal and distal regions of larval caeca. The AaeAPN2 protein binds Cry11Aa with high affinity, 8.6 nM. The full-length and fragments of AaeAPN2 were cloned and expressed in Escherichia coli. The toxin-binding region was identified and further competitive assays demonstrated that Cry11Aa binding to BBMV was efficiently competed by the full-length AaeAPN2 and the fragments of AaeAPN2b and AaeAPN2e. In bioassays against Ae. aegypti larvae, the presence of full-length and a partial fragment (AaeAPN2b) of AaeAPN2 enhanced Cry11Aa larval mortality. Taken together, we conclude that AaeAPN2 is a binding protein and plays a role in Cry11Aa toxicity.
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影响因子：
--
作者：
Abdullah MA;Valaitis AP;Dean DH
通讯作者：
Dean DH
DOI：
10.1152/ajprenal.00487.2005
发表时间：
2007-05-01
影响因子：
4.2
作者：
Kang'ethe, Wanyoike;Aimanova, Karlygash G.;Gill, Sarjeet S.
通讯作者：
Gill, Sarjeet S.
影响因子：
3.8
作者：
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通讯作者：
Gill, Sarjeet S.
影响因子：
4.4
作者：
Atsumi, S;Mizuno, E;Sato, R
通讯作者：
Sato, R
影响因子：
4.8
作者：
GILL, SS;COWLES, EA;FRANCIS, V
通讯作者：
FRANCIS, V