Distribution of Virulence Factors in Escherichia coli Isolated from Urine of Cystitis Patients
Distribution of Virulence Factors in Escherichia coli Isolated from Urine of Cystitis Patients
复制标题
膀胱炎患者尿液中大肠杆菌毒力因子的分布
DOI：
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发表时间：
1995
影响因子：
2.6
通讯作者：
O. Yoshida
中科院分区：
文献类型：
--
作者：
Shingo Yamamoto;T. Tsukamoto;A. Terai;H. Kurazono;Y. Takeda;O. Yoshida
用DNA菌落杂交方法检测了194株膀胱炎患者尿液中分离的大肠埃希菌和80株健康成人粪便标本中I型菌毛(PIL)、肾盂肾炎相关菌毛(PAP)、S菌毛(SFA)、纤毛粘附素I(AFAI)、溶血素(Hly)、需氧蛋白(Aer)和细胞毒性坏死因子1(CNF1)等7种尿毒力因子的分布。除pIL外，所检测的所有毒力因子在膀胱炎分离株中的检出频率明显高于粪便分离株。当单独的毒力因子与其他毒力因子进行分析时，可以看出一种关联，而当所有7种毒力因子被综合考虑时，这种关联并不明显。膀胱炎患者大肠埃希氏菌的基因分型与血清型之间存在明显的相关性。根据所提供的数据，毒力因子的遗传检测将有助于膀胱炎的快速诊断，特别是在没有严重脓尿或菌尿的患者中。
The distribution of 7 urovirulence factors, such as type 1 pilus (pil), pilus associated with pyelonephritis (pap), S fimbriae (sfa), afimbrial adhesin I (afaI), hemolysin (hly), aerobactin (aer) and cytotoxic necrotizing factor 1 (cnf1) was examined by a DNA colony hybridization test among 194 Escherichia coli strains isolated from the urine of cystitis patients and in 80 strains isolated from the stool specimens of healthy adults. All virulence factors examined, except pil, were significantly more frequently detected among the cystitis isolates than among the fecal isolates. When individual virulence factors were analyzed against the others, an association was discernible which was not apparent when all 7 virulence factors were considered collectively. There was an apparent correlation between the genotypes and serotypes of the E. coli strains from the cystitis patients. From the data presented, it was proposed that genetic detection of virulence factors would be useful for rapid diagnosis of cystitis, especially in patients without severe pyuria or bacteriuria.
P-菌毛大肠杆菌在尿路感染中的致病意义。
DOI：
10.1016/s0022-5347(17)49341-4
发表时间：
1985
期刊：
The Journal of urology
影响因子：
--
作者：
Domingue,GJ;Roberts,JA;Laucirica,R;Ratner,MH;Bell,DP;Suarez,GM;Kallenius,G;Svenson,S
通讯作者：
Svenson,S
通过 DNA 菌落杂交检测产肠毒素大肠杆菌。
DOI：
10.1093/infdis/142.6.892
发表时间：
1980
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Moseley,SL;Huq,I;Alim,AR;So,M;Samadpour-Motalebi,M;Falkow,S
通讯作者：
Falkow,S