Abnormal expression of CDK11p58 in prostate cancer.
Abnormal expression of CDK11p58 in prostate cancer.
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DOI：
10.1186/1475-2867-14-2
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发表时间：
2014-01-08
影响因子：
5.8
通讯作者：
Zhou X
中科院分区：
文献类型：
--
作者：
Chi Y;Wang L;Xiao X;Wei P;Wang Y;Zhou X
CDK 11 p58是p34 cdc 2相关激酶的大家族之一，其功能与细胞周期进展、肿瘤发生和凋亡信号相关。我们的前期研究表明CDK 11 p58抑制雄激素受体（AR）的转录活性，并参与AR功能的负调控。采用免疫组化和qRT-PCR检测前列腺癌组织及癌旁组织中CDK 11 p58的表达。流式细胞仪检测细胞凋亡。通过Transwell分析评价癌细胞的转移。最后，我们进一步研究了潜在的分子机制，通过检测相关蛋白质的表达水平，使用蛋白质印迹分析。我们发现，与癌旁组织相比，前列腺癌组织中CDK 11 p58的RNA和蛋白表达均较低。CDK 11 p58以激酶依赖的方式促进前列腺癌细胞凋亡并抑制其转移。CDK 11 p58可通过抑制整合素β3和MMP 2的表达而抑制AR阳性前列腺癌细胞的转移。这些数据表明，CDK 11 p58是前列腺癌中的抗转移基因产物。
CDK11p58 is one of the large families of p34cdc2-related kinases whose functions are linked with cell cycle progression, tumorigenesis and apoptotic signaling. Our previous investigation demonstrated that CDK11p58 repressed androgen receptor (AR) transcriptional activity and was involved in the negative regulation of AR function. CDK11p58 expression was examined in the prostate cancer tissues and adjacent tissues by IHC and qRT-PCR. Cell apoptosis was detected by flow cytometry. The metastasis of cancer cells was evaluated by the Transwell Assay. Finally we further investigated the underlying molecular mechanisms by examining expression levels of relevant proteins using western blot analysis. We found that both RNA and protein expression of CDK11p58 were low in prostate cancer tissues compared with its adjacent noncancerous tissues. CDK11p58 promoted the prostate cancer cell apoptosis and inhibited its metastasis in a kinase dependent way. And finally CDK11p58 could inhibit the metastasis of AR positive prostate cancer cells through inhibition of integrin β3 and MMP2. These data indicate that CDK11p58 is an anti-metastasis gene product in prostate cancer.
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影响因子：
5.3
作者：
Zong, Hongliang;Chi, Yayun;Gu, Jianxin
通讯作者：
Gu, Jianxin
影响因子：
5.7
作者：
Wang X;Lee SO;Xia S;Jiang Q;Luo J;Li L;Yeh S;Chang C
通讯作者：
Chang C
影响因子：
2.8
作者：
Levesque, Marie-Helene;El-Alfy, Mohamed;Labrie, Fernand
通讯作者：
Labrie, Fernand
DOI：
10.5534/wjmh.2013.31.1.36
发表时间：
2013-04
期刊：
The world journal of men's health
影响因子：
--
作者：
Jung SJ;Oh S;Lee GT;Chung J;Min K;Yoon J;Kim W;Ryu DS;Kim IY;Kang DI
通讯作者：
Kang DI
肝雄激素受体通过调节细胞迁移和厌氧菌来抑制肝细胞癌转移。
影响因子：
13.5
作者：
Ma, Wen-Lung;Hsu, Cheng-Lung;Yeh, Chun-Chieh;Wu, Ming-Heng;Huang, Chiung-Kuei;Jeng, Long-Bin;Hung, Yao-Ching;Lin, Tze-Yi;Yeh, Shuyuan;Chang, Chawnshang
通讯作者：
Chang, Chawnshang