Functional Insights of Salinity Stress-Related Pathways in Metagenome-Resolved Methanothrix Genomes.
Functional Insights of Salinity Stress-Related Pathways in Metagenome-Resolved Methanothrix Genomes.
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DOI：
10.1128/aem.02449-21
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发表时间：
2022-05-24
影响因子：
4.4
通讯作者：
Ziels, Ryan M.
中科院分区：
文献类型：
--
作者：
Gagliano, Maria Cristina;Sampara, Pranav;Plugge, Caroline M.;Temmink, Hardy;Sudmalis, Dainis;Ziels, Ryan M.
关键词：
近年来，据报道，产甲烷古菌属甲烷thrix在不同配置/条件下维持厌氧生物反应器中稳定的生态系统功能中起着重要作用。在这项研究中，我们从盐水上流式厌氧污泥毯（UASB）反应器收集的颗粒污泥中重建了三个甲烷thrix宏基因组组装基因组（MAGs），其中，在升高的盐度水平（高达20 g/L Na+）下，甲烷thrix harundinacea与致密稳定颗粒的形成有关。对甲烷菌的基因组注释和途径分析揭示了支持其在高盐度条件下生长的遗传库。其中，最具优势的Methanothrix （MAG_279）被归类为Methanothrix_A harundinacea_D的一个亚种，它有可能通过n -糖基化过程产生不同的糖缀合物，并通过产生相容的溶质如nε -乙酰-β-赖氨酸和外托氨酸来增强其耐盐性。通过生产类异戊二烯来稳定和增强细胞膜被认为是这种微生物中另一个与应激相关的途径。对M. harundinacea盐胁迫相关机制的进一步了解，突出了其在极端条件下的生态位，为高盐环境下有机废物的高效甲烷化以及这种产甲烷菌在高盐自然厌氧环境中可能的持久性开辟了新的视角。利用以基因组为中心的宏基因组学，我们发现了一种新的Methanothrix harundinacea亚种，它似乎是一种耐盐的醋酸分解产甲烷菌，在低（5 g/L Na+）和高盐度（20 g/L Na+）条件下都能灵活地适应厌氧消化过程。对恢复的海龙藻基因组的注释揭示了与盐度胁迫相关的功能，包括EPS糖缀合物的修饰和相容溶质的产生。这是首次报道Methanothrix sp.中这些基因组特征的研究，这是一个里程碑，进一步支持了先前的研究，即M. harundinacea是高盐度胁迫下厌氧造粒的关键驱动因素。
Recently, methanogenic archaea belonging to the genus Methanothrix were reported to have a fundamental role in maintaining stable ecosystem functioning in anaerobic bioreactors under different configurations/conditions. In this study, we reconstructed three Methanothrix metagenome-assembled genomes (MAGs) from granular sludge collected from saline upflow anaerobic sludge blanket (UASB) reactors, where Methanothrix harundinacea was previously implicated with the formation of compact and stable granules under elevated salinity levels (up to 20 g/L Na+). Genome annotation and pathway analysis of the Methanothrix MAGs revealed a genetic repertoire supporting their growth under high salinity. Specifically, the most dominant Methanothrix (MAG_279), classified as a subspecies of Methanothrix_A harundinacea_D, had the potential to augment its salinity resistance through the production of different glycoconjugates via the N-glycosylation process, and via the production of compatible solutes as Nε-acetyl-β-lysine and ectoine. The stabilization and reinforcement of the cell membrane via the production of isoprenoids was identified as an additional stress-related pathway in this microorganism. The improved understanding of the salinity stress-related mechanisms of M. harundinacea highlights its ecological niche in extreme conditions, opening new perspectives for high-efficiency methanisation of organic waste at high salinities, as well as the possible persistence of this methanogen in highly-saline natural anaerobic environments. IMPORTANCE Using genome-centric metagenomics, we discovered a new Methanothrix harundinacea subspecies that appears to be a halotolerant acetoclastic methanogen with the flexibility for adaptation in the anaerobic digestion process both at low (5 g/L Na+) and high salinity conditions (20 g/L Na+). Annotation of the recovered M. harundinacea genome revealed salinity stress-related functions, including the modification of EPS glycoconjugates and the production of compatible solutes. This is the first study reporting these genomic features within a Methanothrix sp., a milestone further supporting previous studies that identified M. harundinacea as a key-driver in anaerobic granulation under high salinity stress.
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影响因子：
6.4
作者：
Clum A;Huntemann M;Bushnell B;Foster B;Foster B;Roux S;Hajek PP;Varghese N;Mukherjee S;Reddy TBK;Daum C;Yoshinaga Y;O'Malley R;Seshadri R;Kyrpides NC;Eloe-Fadrosh EA;Chen IA;Copeland A;Ivanova NN
通讯作者：
Ivanova NN
影响因子：
2.7
作者：
Delmont TO;Eren AM
通讯作者：
Eren AM
影响因子：
4.4
作者：
Aldridge, Jared;Carr, Sean;Buan, Nicole R.
通讯作者：
Buan, Nicole R.
DOI：
10.1007/s10295-015-1632-7
发表时间：
2015-08-01
影响因子：
3.4
作者：
Chen, Si;He, Qiang
通讯作者：
He, Qiang
影响因子：
12.8
作者：
Gagliano, M. C.;Ismail, S. B.;Van Lier, J. B.
通讯作者：
Van Lier, J. B.