The AMP-Activated Protein Kinase Homolog Snf1 Concerts Carbon Utilization, Conidia Production and the Biosynthesis of Secondary Metabolites in the Taxol-Producer Pestalotiopsis microspora.
The AMP-Activated Protein Kinase Homolog Snf1 Concerts Carbon Utilization, Conidia Production and the Biosynthesis of Secondary Metabolites in the Taxol-Producer Pestalotiopsis microspora.
复制标题
AMP 激活的蛋白激酶同系物 Snf1 促进紫杉醇生产者小孢多盘拟盘菌的碳利用、分生孢子产生和次生代谢产物的生物合成
作者：
Wang D;Li Y;Wang H;Wei D;Akhberdi O;Liu Y;Xiang B;Hao X;Zhu X
高度保守的Snf1/AMPK是真核生物中碳代谢和能量产生的核心调节因子。然而，其在丝状真菌中的功能尚未明确。在本研究中，我们报道了Snf1/AMPK在丝状真菌小孢拟盘多毛孢的生长、发育和次生代谢中的功能特性。通过缺失酵母SNF1同源基因，我们发现它调节碳源（例如蔗糖）的利用，这表明该激酶在丝状真菌中具有保守功能。重要的是，揭示了SNF1的一些新功能。例如，缺失菌株在营养生长和色素沉着方面表现出显著的迟缓，并且即使在存在主要碳源葡萄糖的情况下，产生的分生孢子数量也减少。该基因的缺失导致细胞壁受损，这表现为其对荧光增白剂和刚果红的高度敏感性，表明Snf1在维持细胞壁完整性方面具有关键作用。此外，突变菌株Δsnf1对胁迫（例如渗透压（1M山梨醇）、药物G418和热激）高度敏感，尽管其机制仍有待阐明。重要的是，该基因的破坏改变了次生代谢产物的产生。通过高效液相色谱（HPLC）分析，我们发现Δsnf1几乎不产生次生代谢产物，例如已知产物盘长孢内酯B。本研究表明，Snf1是丝状真菌小孢拟盘多毛孢中协调碳代谢以及丝状生长、分生孢子形成、细胞壁完整性、胁迫耐受性和次生代谢产物生物合成的关键调节因子。
Highly conserved, the Snf1/AMPK is a central regulator of carbon metabolism and energy production in the eukaryotes. However, its function in filamentous fungi has not been well established. In this study, we reported functional characterization of Snf1/AMPK in the growth, development and secondary metabolism in the filamentous fungus Pestalotiopsis microspora. By deletion of the yeast SNF1 homolog, we found that it regulated the utilization of carbon sources, e.g., sucrose, demonstrating a conserved function of this kinase in filamentous fungus. Importantly, several novel functions of SNF1 were unraveled. For instance, the deletion strain displayed remarkable retardation in vegetative growth and pigmentation and produced a diminished number of conidia, even in the presence of the primary carbon source glucose. Deletion of the gene caused damages in the cell wall as shown by its hypersensitivities to Calcofluor white and Congo red, suggesting a critical role of Snf1 in maintaining cell wall integrity. Furthermore, the mutant strain Δsnf1 was hypersensitive to stress, e.g., osmotic pressure (1 M sorbitol), drug G418 and heat shock, though the mechanism remains to be illustrated. Significantly, disruption of the gene altered the production of secondary metabolites. By high-performance liquid chromatography (HPLC) profiling, we found that Δsnf1 barely produced secondary metabolites, e.g., the known product pestalotiollide B. This study suggests that Snf1 is a key regulator in filamentous fungus Pestalotiopsis microspora concerting carbon metabolism and the filamentous growth, conidiation, cell wall integrity, stress tolerance and the biosynthesis of secondary metabolites.
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4.8
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