The APC gene, responsible for familial adenomatous polyposis, is mutated in human gastric cancer.
The APC gene, responsible for familial adenomatous polyposis, is mutated in human gastric cancer.
复制标题
导致家族性腺瘤性息肉病的 APC 基因在人类胃癌中发生突变。
作者：
A. Horii;S. Nakatsuru;Y. Miyoshi;S. Ichii;Hiroki Nagase;Y. Kato;A. Yanagisawa;Y. Nakamura
虽然胃癌是世界上最常见的癌症，但其发生过程中的基因变化尚不清楚。由于一些胃癌被认为起源于肠上皮化生，因此腺瘤性息肉病结肠(APC)基因很可能与胃癌的发生有关，该基因的突变导致结肠腺瘤性息肉。基于这一想法，从胃癌中提取的DNA通过RNase保护分析和聚合酶链式反应相结合的方法进行检测，然后对聚合酶链式反应产物进行测序。通过在44个肿瘤中筛选近一半的APC基因编码区，在三个肿瘤中检测到体细胞突变：错义突变、无义突变和5个碱基对缺失导致的框架移位，从而导致基因产物截断。这些结果表明，APC基因的突变在至少部分胃癌的发生过程中也起着重要作用。
Although gastric cancer is the most common cancer in the world, genetic changes during its carcinogenesis are not well understood. Since some gastric cancers are considered to originate from the intestinal metaplasia, it is likely that the adenomatous polyposis coli (APC) gene, the mutation of which causes adenomatous polyps in the colon, is associated with carcinogenesis of gastric cancer. Based on this idea, DNAs isolated from gastric cancers were examined by means of a RNase protection analysis coupled with polymerase chain reaction followed by sequencing of the polymerase chain reaction products. By screening nearly one-half of the coding region of the APC gene in 44 tumors, somatic mutations were detected in three tumors: a missense mutation, a nonsense mutation, and a 5-base pair deletion resulting in a frame shift which causes truncation of the gene product. These results suggest that the mutation of the APC gene also plays an important role during the carcinogenesis of at least some gastric cancers.
登录
查看更多内容
影响因子：
56.9
作者：
NISHISHO, I;NAKAMURA, Y;VOGELSTEIN, B
通讯作者：
VOGELSTEIN, B
DOI：
10.1016/0006-291x(85)90390-0
发表时间：
1985-01-01
影响因子：
3.1
作者：
GOELZ, SE;HAMILTON, SR;VOGELSTEIN, B
通讯作者：
VOGELSTEIN, B
影响因子：
56.9
作者：
KINZLER, KW;NILBERT, MC;NAKAMURA, Y
通讯作者：
NAKAMURA, Y
影响因子：
56.9
作者：
KINZLER, KW;NILBERT, MC;NAKAMURA, Y
通讯作者：
NAKAMURA, Y