Changes in white adipose tissue metabolism induced by resveratrol in rats.
Changes in white adipose tissue metabolism induced by resveratrol in rats.
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DOI：
10.1186/1743-7075-8-29
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发表时间：
2011-05-10
影响因子：
4.5
通讯作者：
Portillo MP
中科院分区：
文献类型：
--
作者：
Alberdi G;Rodríguez VM;Miranda J;Macarulla MT;Arias N;Andrés-Lacueva C;Portillo MP
白藜芦醇具有一系列显著的生物学功能。最近，这种多酚被证明具有降脂作用。本研究的目的是评估在脂肪组织中发生的一些潜在的潜在作用机制。16只雄性SD大鼠随机分为两组：对照组和白藜芦醇30 mg/kg体重/d组，所有大鼠均喂饲肥胖饲料，治疗6周后解剖白色脂肪组织。用荧光法测定脂蛋白脂酶活性，用放免法测定乙酰辅酶A羧基酶活性，用分光光度法测定苹果酸酶、葡萄糖-6P脱氢酶和脂肪酸合成酶的活性。实时定量RT-PCR检测乙酰辅酶A羧基酶、脂肪酸合成酶、脂蛋白脂肪酶、激素敏感脂肪酶、脂肪甘油三酯脂肪酶、PPAR-γ、SREBP-1c和Perilipin的基因表达水平。用层析法测定脂肪组织中白藜芦醇代谢产物的含量。两组大鼠的最终体重没有差异，但白藜芦醇组的脂肪组织明显减少。白藜芦醇可降低造脂酶和肝素释放脂蛋白脂肪酶的活性。此外，这种多酚显著降低了激素敏感脂肪酶的mRNA水平。其他基因未见明显变化。脂肪组织中白藜芦醇代谢物总量为2.66±0.55nmol/g组织。可以认为，白藜芦醇的降脂作用至少部分是通过减少循环中三酰甘油的脂肪酸摄取以及新生脂肪生成来实现的。
A remarkable range of biological functions have been ascribed to resveratrol. Recently, this polyphenol has been shown to have body fat lowering effects. The aim of the present study was to assess some of the potential underlying mechanisms of action which take place in adipose tissue. Sixteen male Sprague-Dawley rats were randomly divided into two groups: control and treated with 30 mg resveratrol/kg body weight/d. All rats were fed an obesogenic diet and after six weeks of treatment white adipose tissues were dissected. Lipoprotein lipase activity was assessed by fluorimetry, acetyl-CoA carboxylase by radiometry, and malic enzyme, glucose-6P-dehydrogenase and fatty acid synthase by spectrophotometry. Gene expression levels of acetyl-CoA carboxylase, fatty acid synthase, lipoprotein lipase, hormone-sensitive lipase, adipose triglyceride lipase, PPAR-gamma, SREBP-1c and perilipin were assessed by Real time RT-PCR. The amount of resveratrol metabolites in adipose tissue was measured by chromatography. There was no difference in the final body weight of the rats; however, adipose tissues were significantly decreased in the resveratrol-treated group. Resveratrol reduced the activity of lipogenic enzymes, as well as that of heparin-releasable lipoprotein lipase. Moreover, a significant reduction was induced by this polyphenol in hormone-sensitive lipase mRNA levels. No significant changes were observed in other genes. Total amount of resveratrol metabolites in adipose tissue was 2.66 ± 0.55 nmol/g tissue. It can be proposed that the body fat-lowering effect of resveratrol is mediated, at least in part, by a reduction in fatty acid uptake from circulating triacylglycerols and also in de novo lipogenesis.
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DOI：
10.1111/j.1432-1033.1990.tb15592.x
发表时间：
1990-06-20
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
KATSURADA, A;IRITANI, N;TANAKA, T
通讯作者：
TANAKA, T
影响因子：
8.2
作者：
Shang, Jing;Chen, Lu-lu;Xiao, Hu
通讯作者：
Xiao, Hu
影响因子：
7.1
作者：
Fischer-Posovszky, Pamela;Kukulus, Vera;Wabitsch, Martin
通讯作者：
Wabitsch, Martin
影响因子：
3.9
作者：
Munday, MR
通讯作者：
Munday, MR
DOI：
10.1111/j.1749-6632.2010.05845.x
发表时间：
2011-01-01
期刊：
RESVERATROL AND HEALTH
影响因子：
--
作者：
Baile, Clifton A.;Yang, Jeong-Yeh;Della-Fera, Mary Anne
通讯作者：
Della-Fera, Mary Anne