Foam nest components of the túngara frog: a cocktail of proteins conferring physical and biological resilience.
Foam nest components of the túngara frog: a cocktail of proteins conferring physical and biological resilience.
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DOI：
10.1098/rspb.2008.1939
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发表时间：
2009-05-22
期刊：
影响因子：
--
通讯作者：
Kennedy MW
中科院分区：
文献类型：
--
作者：
Fleming RI;Mackenzie CD;Cooper A;Kennedy MW
唐加拉蛙（Engystomops pustulosus）的泡沫巢形成了卵子和精子的生物相容性孵化培养基，同时抵抗相当大的环境和微生物攻击。我们已经证明，这种行为的大部分可以归因于六种蛋白质的混合物，称为ranaspumins（Rsn-1至Rsn-6），它们在泡沫中占主导地位。根据序列分析和生物物理性质，这些基因可分为两类。Rsn-2，与任何迄今为止报道的两亲性氨基酸序列不同，表现出大量的洗涤剂样表面活性剂活性所需的泡沫的生产，但对卵和精子的膜是无害的。另外四种（Rsn-3至Rsn-6）是凝集素，其中三种类似于硬骨鱼中发现的岩藻凝集素，但先前未在陆地脊椎动物中鉴定出，尽管具有与先前描述的岩藻凝集素不同的碳水化合物结合特异性。第六个，Rsn-1，在结构上类似于半胱氨酸蛋白酶抑制剂类的蛋白酶抑制剂，但本身似乎没有表现出任何这样的活性。然而，巢泡沫本身确实表现出有效的半胱氨酸蛋白酶抑制剂活性。rsn编码基因在成年青蛙的许多组织中转录，但完整的鸡尾酒只存在于输卵管腺中。凝集素和半胱氨酸蛋白酶抑制剂的组合在植物和动物中具有已知的防御微生物定殖和昆虫攻击的作用。Túngara巢泡沫显示出一种新的协同作用，选择先天防御的元素加上一种专门的表面活性剂蛋白，包括一种以前未报道的保护动物无人看管的生殖阶段的策略。
The foam nests of the túngara frog (Engystomops pustulosus) form a biocompatible incubation medium for eggs and sperm while resisting considerable environmental and microbiological assault. We have shown that much of this behaviour can be attributed to a cocktail of six proteins, designated ranaspumins (Rsn-1 to Rsn-6), which predominate in the foam. These fall into two discernable classes based on sequence analysis and biophysical properties. Rsn-2, with an amphiphilic amino acid sequence unlike any hitherto reported, exhibits substantial detergent-like surfactant activity necessary for production of foam, yet is harmless to the membranes of eggs and spermatozoa. A further four (Rsn-3 to Rsn-6) are lectins, three of which are similar to fucolectins found in teleosts but not previously identified in a land vertebrate, though with a carbohydrate binding specificity different from previously described fucolectins. The sixth, Rsn-1, is structurally similar to proteinase inhibitors of the cystatin class, but does not itself appear to exhibit any such activity. The nest foam itself, however, does exhibit potent cystatin activity. Rsn-encoding genes are transcribed in many tissues of the adult frogs, but the full cocktail is present only in oviduct glands. Combinations of lectins and cystatins have known roles in plants and animals for defence against microbial colonization and insect attack. Túngara nest foam displays a novel synergy of selected elements of innate defence plus a specialized surfactant protein, comprising a previously unreported strategy for protection of unattended reproductive stages of animals.
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影响因子：
3.4
作者：
Cooper, A;Kennedy, MW;Lu, JR
通讯作者：
Lu, JR
DOI：
10.1038/nsb817
发表时间：
2002-08-01
期刊：
NATURE STRUCTURAL BIOLOGY
影响因子：
--
作者：
Bianchet, MA;Odom, EW;Amzel, LM
通讯作者：
Amzel, LM
影响因子：
14.9
作者：
CORPET, F
通讯作者：
CORPET, F
影响因子：
4.1
作者：
BEELEY, JG;EASON, R;SNOW, DH
通讯作者：
SNOW, DH
影响因子：
2.8
作者：
Hissa, Denise Cavalcante;Vasconcelos, Ilka Maria;Maciel Melo, Vania Maria
通讯作者：
Maciel Melo, Vania Maria