Spaciotemporal Alteration of 8-hydroxy-2′-deoxyguanosine Levels in Cardiomyocytes After Myocardial Infarction in Rats
Spaciotemporal Alteration of 8-hydroxy-2′-deoxyguanosine Levels in Cardiomyocytes After Myocardial Infarction in Rats
复制标题
大鼠心肌梗死后心肌细胞8-羟基-2-脱氧鸟苷水平的时空变化
DOI：
10.1081/1071576021000005285
复制
发表时间：
2002
影响因子：
3.3
通讯作者：
M. Komeda
中科院分区：
文献类型：
--
作者：
S. Miwa;S. Toyokuni;T. Nishina;T. Nomoto;M. Hiroyasu;K. Nishimura;M. Komeda
暂时性或持续性心力衰竭是心肌梗死后的主要并发症之一。为了阐明心肌梗死的发病机制，我们研究了结扎大鼠冠状动脉左前降支分支后心肌细胞8-羟基脱氧鸟苷（8-OHdG）水平的时空变化。该模型的致死率为18%。分别于术后0、3、6、12、24、48 h和1、2、4、6周解剖心脏。用生化方法和单克隆抗体N45.1免疫组化方法评价8-OHdG的心脏水平。结扎后3 ~ 6 h，MI区和MI周围区心肌细胞8-OHdG含量分别增加6倍和4倍。24 h后，MI区心肌坏死，8-OHdG水平下降。8-心肌梗死周围区域的心肌中的OHdG水平一度恢复到正常水平，但在沿着该区域中凋亡心肌细胞的出现而显著增加。MI后的心脏通常被认为在4周后临床稳定。然而，即使在四周后，当受影响的病变广泛时，梗死区附近的心肌细胞也受到氧化应激。目前的数据支持使用补充抗氧化剂治疗来挽救MI后的功能性心肌。(213字）
Temporary or persistent heart failure is one of the major complications after myocardial infarction (MI). In order to elucidate the pathogenesis of MI, we studied the spaciotemporal alteration of 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels in cardiomyocytes in a rat model of ligation of the left anterior descending branch of the coronary artery. The lethality in this model was 18%. Hearts were dissected at 0, 3, 6, 12, 24, 48 h, and 1, 2, 4, 6 weeks after the operation. The cardiac level of 8-OHdG was evaluated biochemically as well as by immunohistochemistry with monoclonal antibody N45.1. Three to 6 h after ligation, the 8-OHdG levels were increased in the cardiomyocytes of MI (six-fold) and peri-MI (four-fold) areas. After 24 h, the myocardium in the MI area was necrotized, and thereafter the 8-OHdG level decreased. 8-OHdG levels in the myocardium of peri-MI areas returned once to a normal level, but were significantly increased at 2-4 weeks along with the appearance of apoptotic cardiomyocytes in this area. The heart after MI has been generally considered as clinically stable after four weeks. However, cardiomyocytes near the infarcted area were oxidatively stressed even after four weeks when the affected lesion was extensive. The present data support the use of supplementary antioxidant therapies to save functional myocardium after MI. (213 words)
影响因子：
3.2
作者：
G. Simone;D. Wallerson;Massimo Volpe;R. B. Devereux
通讯作者：
G. Simone;D. Wallerson;Massimo Volpe;R. B. Devereux
影响因子：
37.8
作者：
PFEFFER, MA;PFEFFER, JM;FINN, P
通讯作者：
FINN, P
影响因子：
8.3
作者：
Soriano, SG;Coxon, A;Mayadas, TN
通讯作者：
Mayadas, TN