Novel insights into the effect of nitrogen on storage protein biosynthesis and protein body development in wheat caryopsis
Novel insights into the effect of nitrogen on storage protein biosynthesis and protein body development in wheat caryopsis
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关于氮对小麦颖果储存蛋白生物合成和蛋白体发育影响的新见解
DOI：
10.1093/jxb/erx108
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发表时间：
2017-04
影响因子：
6.9
通讯作者：
Xiong Fei
中科院分区：
文献类型：
--
作者：
Yu Xurun;Chen Xinyu;Wang Leilei;Yang Yang;Zhu Xiaowei;Shao Shanshan;Cui Wenxue;Xiong Fei
小麦氮素效应的分子和细胞学机制贮藏蛋白质生物合成和蛋白体发育仍然很难预测。我们使用转录组测序，蛋白质组学技术和光学显微镜来研究这些问题。开花后7 d共检测到2585个差异表达基因（DEG）和57个差异表达蛋白（DEPs），施氮后18 d共检测到2456个差异表达基因（DEG）和64个差异表达蛋白。与蛋白质生物合成过程相关的基因本体论术语在7和18 DAA分别使这些数字增加了678和582 DEG。此外，25个京都基因百科全书和基因组途径在7和18 DAA参与蛋白质生物合成。与贮藏蛋白生物合成相关的DEPs包括醇溶蛋白和麦谷蛋白亚基，其中大部分在氮处理后表达上调。实时荧光定量PCR分析表明，一些醇溶蛋白和麦谷蛋白亚基编码基因在花后18天差异表达。结构观察表明，施氮处理后小麦胚乳蛋白体积累较多，体积较大。总的来说，我们的研究结果表明，氮处理通过改变蛋白质生物合成途径和蛋白质组学水平上的贮藏蛋白表达中涉及的某些基因的表达水平来提高贮藏蛋白含量、胚乳蛋白体数量和部分加工质量。
Molecular and cytological mechanisms concerning the effects of nitrogen on wheat (Triticum aestivum L.) storage protein biosynthesis and protein body development remain largely elusive. We used transcriptome sequencing, proteomics techniques, and light microscopy to investigate these issues. In total, 2585 differentially expressed genes (DEGs) and 57 differentially expressed proteins (DEPs) were found 7 days after anthesis (DAA), and 2456 DEGs and 64 DEPs were detected 18 DAA after nitrogen treatment. Gene ontology terms related to protein biosynthesis processes enriched these numbers by 678 and 582 DEGs at 7 and 18 DAA, respectively. Further, 25 Kyoto Encyclopedia of Genes and Genomes pathways were involved in protein biosynthesis at both 7 and 18 DAA. DEPs related to storage protein biosynthesis contained gliadin and glutenin subunits, most of which were up-regulated after nitrogen treatment. Quantitative real-time PCR analysis indicated that some gliadin and glutenin subunit encoding genes were differentially expressed at 18 DAA. Structural observation revealed that wheat endosperm accumulated more and larger protein bodies after nitrogen treatment. Collectively, our findings suggest that nitrogen treatment enhances storage protein content, endosperm protein body quantity, and partial processing quality by altering the expression levels of certain genes involved in protein biosynthesis pathways and storage protein expression at the proteomics level.
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影响因子：
64.5
作者：
Buszczak M;Signer RA;Morrison SJ
通讯作者：
Morrison SJ
影响因子：
5.6
作者：
Zhang N;Chen F;Huo W;Cui D
通讯作者：
Cui D
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
通讯作者：
--
影响因子：
6.9
作者：
Plessis A;Ravel C;Bordes J;Balfourier F;Martre P
通讯作者：
Martre P
影响因子：
6.1
作者：
Godfrey, Dan;Hawkesford, Malcolm J.;Shewry, Peter R.
通讯作者：
Shewry, Peter R.