Mrc1-Dependent Chromatin Compaction Represses DNA Double-Stranded Break Repair by Homologous Recombination Upon Replication Stress.
Mrc1-Dependent Chromatin Compaction Represses DNA Double-Stranded Break Repair by Homologous Recombination Upon Replication Stress.
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Mrc1 依赖性染色质压缩通过复制应激时的同源重组抑制 DNA 双链断裂修复
DOI：
10.3389/fcell.2021.630777
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发表时间：
2021
影响因子：
5.5
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Xing P;Dong Y;Zhao J;Zhou Z;Li Z;Wang Y;Li M;Zhang X;Chen X
The coordination of DNA replication and repair is critical for the maintenance of genome stability. It has been shown that the Mrc1-mediated S phase checkpoint inhibits DNA double-stranded break (DSB) repair through homologous recombination (HR). How the replication checkpoint inhibits HR remains only partially understood. Here we show that replication stress induces the suppression of both Sgs1/Dna2- and Exo1-mediated resection pathways in an Mrc1-dependent manner. As a result, the loading of the single-stranded DNA binding factor replication protein A (RPA) and Rad51 and DSB repair by HR were severely impaired under replication stress. Notably, the deletion of MRC1 partially restored the recruitment of resection enzymes, DSB end resection, and the loading of RPA and Rad51. The role of Mrc1 in inhibiting DSB end resection is independent of Csm3, Tof1, or Ctf4. Mechanistically, we reveal that replication stress induces global chromatin compaction in a manner partially dependent on Mrc1, and this chromatin compaction limits the access of chromatin remodeling factors and HR proteins, leading to the suppression of HR. Our study reveals a critical role of the Mrc1-dependent chromatin structure change in coordinating DNA replication and recombination under replication stress.
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影响因子：
8.8
作者：
Khurana S;Kruhlak MJ;Kim J;Tran AD;Liu J;Nyswaner K;Shi L;Jailwala P;Sung MH;Hakim O;Oberdoerffer P
通讯作者：
Oberdoerffer P
影响因子：
3.8
作者：
Cortez D
通讯作者：
Cortez D
影响因子：
64.8
作者：
Costelloe, Thomas;Louge, Raphael;Tomimatsu, Nozomi;Mukherjee, Bipasha;Martini, Emmanuelle;Khadaroo, Basheer;Dubois, Kenny;Wiegant, Wouter W.;Thierry, Agnes;Burma, Sandeep;van Attikum, Haico;Llorente, Bertrand
通讯作者：
Llorente, Bertrand
DOI：
10.1007/s00018-017-2474-4
发表时间：
2017-07
期刊：
Cellular and molecular life sciences : CMLS
影响因子：
--
作者：
Giannattasio M;Branzei D
通讯作者：
Branzei D
影响因子：
11.4
作者：
Gonzalez-Prieto, Roman;Munoz-Cabello, Ana M.;Prado, Felix
通讯作者：
Prado, Felix