Entada phaseoloides extract suppresses hepatic gluconeogenesis via activation of the AMPK signaling pathway.
Entada phaseoloides extract suppresses hepatic gluconeogenesis via activation of the AMPK signaling pathway.
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Entada 菜豆提取物通过激活 AMPK 信号通路抑制肝脏糖异生。
DOI：
10.1016/j.jep.2016.10.039
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发表时间：
2016-12
期刊：
影响因子：
--
通讯作者：
Bian Fang
中科院分区：
文献类型：
--
作者：
Zheng Tao;Hao Xincai;Wang Qibin;Chen Li;Jin Si;Bian Fang
三叶草Entada phaseoloides(L.)默尔。相思(Entada Phaseoloides)是我国少数民族治疗糖尿病的一种民间药物。近年来的研究表明，石杉总皂苷(TSEP)具有降低2型糖尿病大鼠空腹血糖的作用。然而，其作用机制尚未完全阐明。本研究旨在探讨TSEP在2型糖尿病(T2 DM)中的作用机制。材料与方法采用原代小鼠肝细胞和HepG2细胞观察TSEP对糖异生的影响。经TSEP处理后，检测葡萄糖生成量、葡萄糖-6-磷酸酶(G6pase)和磷酸烯甲酰丙酮酸羧激酶(PEPCK)基因的表达水平。结果TSEP明显抑制糖生成和糖异生基因的表达。TSEP可促进AMPK的磷酸化，进而促进乙酰辅酶A(Acc)和Akt/糖原合成酶β(GSK3β)的磷酸化。结论TSEP通过AMPK信号转导途径抑制肝脏糖异生发挥抗糖尿病作用。
Ethnopharmacological relevanceThe seed ofEntada phaseoloides(L.) Merr. (Entada phaseoloides) has been long used as a folk medicine for the treatment of Diabetes mellitus by Chinese ethnic minorities. Recent reports have demonstrated that total saponins fromEntada phaseoloides(TSEP) could reduce fasting blood glucose in type 2 diabetic rats. However, the mechanism has not been fully elucidated. The aim of this study was to explore the underlying mechanisms of TSEP on type 2 Diabetes mellitus (T2DM).Materials and methodsPrimary mouse hepatocytes and HepG2 cells were used to investigate the effects of TSEP on gluconeogenesis. After treatment with TSEP, glucose production, genes expression levels ofGlucose-6-phosphatase(G6pase) andPhosphoenoylpyruvate carboxykinase(Pepck) were detected. The efficacy and underlying mechanism of TSEP on AMP-activated protein kinase (AMPK) signaling pathway were determinated.ResultsTSEP significantly inhibited glucose production and the gluconeogenic gene expression. Treatment with TSEP elevated the phosphorylation of AMPK, which in turn promoted the phosphorylation of acetyl coenzyme A (ACC) and Akt/glycogen synthase kinase 3β (GSK3β), respectively. Furthermore, TSEP reduced lipid accumulation and improved insulin sensitivity in hepatocytes.ConclusionThese findings provide evidence that TSEP exerts an antidiabetic effect by suppressing hepatic gluconeogenesis via the AMPK signaling pathway.
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影响因子：
16.2
作者：
Lomonaco R;Bril F;Portillo-Sanchez P;Ortiz-Lopez C;Orsak B;Biernacki D;Lo M;Suman A;Weber MH;Cusi K
通讯作者：
Cusi K
影响因子：
7.7
作者：
Hardie DG
通讯作者：
Hardie DG
DOI：
10.1042/bj3480607
发表时间：
2000-06
期刊：
The Biochemical journal
影响因子：
--
作者：
Mark R. Owen;E. Doran;A. Halestrap
通讯作者：
Mark R. Owen;E. Doran;A. Halestrap
影响因子：
3.4
作者：
Hui Xiong;Yanan Zheng;Guangzhong Yang;Huixia Wang;Zhinan Mei
通讯作者：
Hui Xiong;Yanan Zheng;Guangzhong Yang;Huixia Wang;Zhinan Mei
DOI：
--
发表时间：
2014-04
期刊：
Journal of B.U.ON. : official journal of the Balkan Union of Oncology
影响因子：
--
作者：
Li Zhang;Ling Huang;Qiang-Ni Liu;Shao-Yi Kuang;Qiao-Qin Xu;Xianjin Qin;S. Fu;Qian Wu;Qibing Liu;Yu-qin Zeng
通讯作者：
Li Zhang;Ling Huang;Qiang-Ni Liu;Shao-Yi Kuang;Qiao-Qin Xu;Xianjin Qin;S. Fu;Qian Wu;Qibing Liu;Yu-qin Zeng