Construction of a high resolution microscope with conventional and holographic optical trapping capabilities.
Construction of a high resolution microscope with conventional and holographic optical trapping capabilities.
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构建具有传统和全息光学捕获功能的高分辨率显微镜。
DOI：
10.3791/50481
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
M. Vershinin
中科院分区：
文献类型：
--
作者：
Jacqualine Butterfield;Weili Hong;Leslie Mershon;M. Vershinin
具有光学陷阱的高分辨率显微镜系统可以精确操纵各种折射物体，例如介电珠 (1) 或细胞器 (2,3)，以及以高空间和时间分辨率读出它们相对于陷阱中心的位置。本文描述的系统具有一种在 980 nm 下工作的此类“传统”陷阱。它还提供了第二个光学捕获系统，该系统使用市售的全息封装在显微镜 (4,5) 的视野中以 1,064 nm 的波长同时创建和操作复杂的捕获图案。两个系统的组合允许同时操纵多个折射物体，同时对纳米和皮牛顿尺度的运动和力产生进行高速和高分辨率测量。
High resolution microscope systems with optical traps allow for precise manipulation of various refractive objects, such as dielectric beads (1) or cellular organelles (2,3), as well as for high spatial and temporal resolution readout of their position relative to the center of the trap. The system described herein has one such "traditional" trap operating at 980 nm. It additionally provides a second optical trapping system that uses a commercially available holographic package to simultaneously create and manipulate complex trapping patterns in the field of view of the microscope (4,5) at a wavelength of 1,064 nm. The combination of the two systems allows for the manipulation of multiple refractive objects at the same time while simultaneously conducting high speed and high resolution measurements of motion and force production at nanometer and piconewton scale.
用于研究分子马达特性的光陷阱。
影响因子：
--
作者：
Spudich,JamesA;Rice,SarahE;Rock,RonaldS;Purcell,ThomasJ;Warrick,HansM
通讯作者：
Warrick,HansM
影响因子：
3.6
作者：
M. Valentine;N. Guydosh;B. Gutiérrez-Medina;Adrian N. Fehr;J. O. Andreasson;S. Block
通讯作者：
M. Valentine;N. Guydosh;B. Gutiérrez-Medina;Adrian N. Fehr;J. O. Andreasson;S. Block