Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes.
Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes.
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DOI：
10.1038/s41467-018-05573-y
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发表时间：
2018-09-07
影响因子：
16.6
通讯作者：
Evseenko D
中科院分区：
文献类型：
--
作者：
Ferguson GB;Van Handel B;Bay M;Fiziev P;Org T;Lee S;Shkhyan R;Banks NW;Scheinberg M;Wu L;Saitta B;Elphingstone J;Larson AN;Riester SM;Pyle AD;Bernthal NM;Mikkola HK;Ernst J;van Wijnen AJ;Bonaguidi M;Evseenko D
Tissue-specific gene expression defines cellular identity and function, but knowledge of early human development is limited, hampering application of cell-based therapies. Here we profiled 5 distinct cell types at a single fetal stage, as well as chondrocytes at 4 stages in vivo and 2 stages during in vitro differentiation. Network analysis delineated five tissue-specific gene modules; these modules and chromatin state analysis defined broad similarities in gene expression during cartilage specification and maturation in vitro and in vivo, including early expression and progressive silencing of muscle- and bone-specific genes. Finally, ontogenetic analysis of freshly isolated and pluripotent stem cell-derived articular chondrocytes identified that integrin alpha 4 defines 2 subsets of functionally and molecularly distinct chondrocytes characterized by their gene expression, osteochondral potential in vitro and proliferative signature in vivo. These analyses provide new insight into human musculoskeletal development and provide an essential comparative resource for disease modeling and regenerative medicine. Human development provides a roadmap for advancing pluripotent stem cell-based regenerative therapies. Here the authors mapped human skeletogenesis using RNA sequencing on 5 cell types from a single foetal stage as well as chondrocytes at 4 stages in vivo and 2 stages during in vitro differentiation.
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DOI：
10.1073/pnas.0504750102
发表时间：
2005-10-11
影响因子：
11.1
作者：
Akiyama, H;Kim, JE;de Crombrugghe, B
通讯作者：
de Crombrugghe, B
影响因子：
4
作者：
Cerletti, Massimiliano;Molloy, Michael J.;Gussoni, Emanuela
通讯作者：
Gussoni, Emanuela
在人中胚层分化过程中的遗传标记揭示了三层侧板中胚层祖细胞。
DOI：
10.1002/stem.2351
发表时间：
2016-05
期刊：
Stem cells (Dayton, Ohio)
影响因子：
--
作者：
Chin CJ;Cooper AR;Lill GR;Evseenko D;Zhu Y;He CB;Casero D;Pellegrini M;Kohn DB;Crooks GM
通讯作者：
Crooks GM
影响因子：
3.5
作者：
Hoshijima, M;Hattori, T;Takigawa, M
通讯作者：
Takigawa, M
影响因子：
11.4
作者：
HOLZMANN, B;WEISSMAN, IL
通讯作者：
WEISSMAN, IL