Effects of N input level on the N-associated traits and physiological processes of winter wheat cultivated under water-saving condition
Effects of N input level on the N-associated traits and physiological processes of winter wheat cultivated under water-saving condition
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氮输入水平对节水条件下冬小麦氮素相关性状及生理过程的影响
DOI：
10.1080/01904167.2020.1783307
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发表时间：
2020-06
影响因子：
2.1
通讯作者：
Kai Xiao
中科院分区：
文献类型：
--
作者：
Ruize Lin;Xinyang Bai;Fangafang Li;Shuang Zhang;Le Han;Kai Xiao
摘要提高谷类作物氮素利用效率(NUE)对全球农业可持续发展具有重要意义。本研究以两个不同的氮素利用效率品种为材料，研究了氮素输入水平对亏缺灌溉条件下冬小麦氮素相关性状和农艺性状的影响。在氮素充足(SN，N240)条件下，氮素利用效率较高的石农086和缺氮敏感的冀麦325两个品种在不同生育期和成熟期的氮素含量、生物量、氮素累积量、光合作用和产量均高于氮素不足处理(SN，N120)。在供试的两个品种中，世农086在SN和ND处理下对冀麦325的氮素相关性状、光合功能和农艺性状都有明显的改善。这些结果与供试小麦品种的供氮反应是一致的。硝酸盐转运蛋白(NRT)家族基因TaNRT1和硝酸还原酶(NR)、亚硝酸盐还原酶(NIR)和谷氨酰胺合成酶(GS)家族基因TaNR5/6、TaNIR6/7和TaGS3/4在两个缺氮品种中的表达水平均显著上调，其中石农086的转录量高于冀麦325。对TaNRT1和TaGS3的转基因分析验证了它们分别对植株氮素吸收和GS活性的正向调节作用。因此，由于转录效率的提高，不同的N吸收和N同化相关基因有助于小麦植株对低N胁迫的植物适应。
Abstract Enhancing nitrogen (N) use efficiency (NUE) of cereal crops contribute largely to the sustainable agriculture worldwide. In this study, the effects of N input level on the N-associated and agronomic traits of winter wheat cultivated under deficit irrigation were investigated, using two contrasting NUE cultivars. Under sufficient-N treatment (SN, N240), the two cultivars including the high NUE Shinong 086 and the N deprivation sensitive Jimai 325 both displayed higher N concentrations, biomass, N accumulative amounts, and photosynthetic function at various growth stages and yields at maturity than those obtained under deficient-N treatment (DN, N120). For two cultivars examined, Shinong 086 showed profound improvement on the N-associated traits, photosynthetic function, and the agronomic traits with respect to Jimai 325 under both SN and ND treatments. These results were in consistent with the N supply responses of the tested wheat cultivars. Expression levels of TaNRT1, a nitrate transporter (NRT) family gene mediating N uptake and TaNR5/6, TaNIR6/7, and TaGS3/4, the genes in nitrate reductase (NR), nitrite reductase (NIR), and glutamine synthetase (GS) families, respectively, were significantly upregulated in both N-deprived cultivars, with more transcripts in Shinong 086 plants than the Jimai 325 ones. Transgene analysis on TaNRT1 and TaGS3 validated their functions in positively regulating the plant N uptake and GS activity under DN conditions, respectively. Thus, distinct N uptake- and N assimilation-associated genes contributes to the plant adaptation of wheat plants to low-N stress given the enhanced transcription efficiency.
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DOI：
10.1016/s1875-2780(09)60079-1
发表时间：
2010-10
期刊：
Acta Agronomica Sinica
影响因子：
--
作者：
Jun-hua Zhang;Jianli Liu;Jiabao Zhang;Fumei Zhao;Ya-Nan Cheng;Wei-Peng Wang
通讯作者：
Jun-hua Zhang;Jianli Liu;Jiabao Zhang;Fumei Zhao;Ya-Nan Cheng;Wei-Peng Wang
DOI：
--
发表时间：
2015-06
期刊：
Ying yong sheng tai xue bao = The journal of applied ecology
影响因子：
--
作者：
Juan Zhang;Tong-hua Wu;X. Dai;Xi-zhi Wang;Hong-mei Li;Ming Jiang;M. He
通讯作者：
Juan Zhang;Tong-hua Wu;X. Dai;Xi-zhi Wang;Hong-mei Li;Ming Jiang;M. He
影响因子：
11.6
作者：
Wang, RC;Guegler, K;Crawford, NM
通讯作者：
Crawford, NM
DOI：
10.3969/j.issn.1002-6819.2011.7.007
发表时间：
2011-07
期刊：
Transactions of the Chinese Society of Agricultural Engineering
影响因子：
--
作者：
Fang Quanxiao;Wang Jianlin;Y. Shunzhang
通讯作者：
Fang Quanxiao;Wang Jianlin;Y. Shunzhang
影响因子：
6.9
作者：
Lawlor, DW
通讯作者：
Lawlor, DW