New insights into the evolution of subtilisin-like serine protease genes in Pezizomycotina.
New insights into the evolution of subtilisin-like serine protease genes in Pezizomycotina.
复制标题
关于盘菌亚门中枯草杆菌蛋白酶样丝氨酸蛋白酶基因进化的新见解
DOI：
10.1186/1471-2148-10-68
复制
发表时间：
2010-03-09
影响因子：
3.4
通讯作者：
Zhang K
中科院分区：
文献类型：
--
作者：
Li J;Yu L;Yang J;Dong L;Tian B;Yu Z;Liang L;Zhang Y;Wang X;Zhang K
研究背景枯草杆菌蛋白酶样丝氨酸蛋白酶在病原真菌侵入宿主和定殖过程中发挥重要作用。本研究通过对盘菌亚门类枯草杆菌蛋白酶丝氨酸蛋白酶基因的进化分析，探讨不同生活方式的病原真菌是否具有相似的致病机制，并将其作为致病因子。在盘菌亚门中，捕食线虫的真菌是独一无二的，因为它们使用专门的诱捕装置来捕捉土壤线虫。越来越多的证据表明，来自捕食线虫真菌的枯草杆菌蛋白酶样丝氨酸蛋白酶参与线虫幼虫的穿透和消化。在这里，我们还进行了积极的选择分析的枯草杆菌蛋白酶样丝氨酸蛋白酶基因从线虫捕捉fungi.Results189枯草杆菌蛋白酶样丝氨酸蛋白酶基因盘菌亚门的系统发育分析表明，5个强有力的支持单系分支。枯草杆菌蛋白酶样丝氨酸蛋白酶基因以前确定或推定为细胞内蛋白酶聚成一个分支，并在进化史上最早分歧。此外，昆虫病原真菌和线虫寄生真菌的表皮降解蛋白酶基因聚在一起，表明它们可能对昆虫和线虫具有重叠的致病机制。我们的实验生物测定支持这一结论。有趣的是，虽然它们都作为表皮降解蛋白酶的功能，枯草杆菌蛋白酶样丝氨酸蛋白酶基因从线虫捕捉真菌和线虫寄生真菌没有分组在一起的系统发育树。我们的进化分析揭示了证据的积极选择的枯草杆菌蛋白酶样丝氨酸蛋白酶基因的线虫诱捕fungi.ConclusionsOur研究提供了新的见解的进化的枯草杆菌蛋白酶样丝氨酸蛋白酶基因在盘菌亚门。盘菌亚门枯草杆菌蛋白酶最可能从细胞内蛋白酶进化到细胞外蛋白酶。昆虫病原真菌和线虫寄生真菌可能具有相似的寄生特性。此外，我们的数据提供了更好的理解的重复和随后的功能分歧的枯草杆菌蛋白酶样丝氨酸蛋白酶基因在盘菌亚门。本研究在捕食线虫真菌类枯草杆菌蛋白酶丝氨酸蛋白酶基因中检测到的正选择证据表明，类枯草杆菌蛋白酶丝氨酸蛋白酶可能在捕食线虫真菌对线虫的致病性进化过程中发挥了重要作用。
BackgroundSubtilisin-like serine proteases play an important role in pathogenic fungi during the penetration and colonization of their hosts. In this study, we perform an evolutionary analysis of the subtilisin-like serine protease genes of subphylum Pezizomycotina to find if there are similar pathogenic mechanisms among the pathogenic fungi with different life styles, which utilize subtilisin-like serine proteases as virulence factors. Within Pezizomycotina, nematode-trapping fungi are unique because they capture soil nematodes using specialized trapping devices. Increasing evidence suggests subtilisin-like serine proteases from nematode-trapping fungi are involved in the penetration and digestion of nematode cuticles. Here we also conduct positive selection analysis on the subtilisin-like serine protease genes from nematode-trapping fungi.ResultsPhylogenetic analysis of 189 subtilisin-like serine protease genes from Pezizomycotina suggests five strongly-supported monophyletic clades. The subtilisin-like serine protease genes previously identified or presumed as endocellular proteases were clustered into one clade and diverged the earliest in the phylogeny. In addition, the cuticle-degrading protease genes from entomopathogenic and nematode-parasitic fungi were clustered together, indicating that they might have overlapping pathogenic mechanisms against insects and nematodes. Our experimental bioassays supported this conclusion. Interestingly, although they both function as cuticle-degrading proteases, the subtilisin-like serine protease genes from nematode-trapping fungi and nematode-parasitic fungi were not grouped together in the phylogenetic tree. Our evolutionary analysis revealed evidence for positive selection on the subtilisin-like serine protease genes of the nematode-trapping fungi.ConclusionsOur study provides new insights into the evolution of subtilisin-like serine protease genes in Pezizomycotina. Pezizomycotina subtilisins most likely evolved from endocellular to extracellular proteases. The entomopathogenic and nematode-parasitic fungi likely share similar properties in parasitism. In addition, our data provided better understanding about the duplications and subsequent functional divergence of subtilisin-like serine protease genes in Pezizomycotina. The evidence of positive selection detected in the subtilisin-like serine protease genes of nematode-trapping fungi in the present study suggests that the subtilisin-like serine proteases may have played important roles during the evolution of pathogenicity of nematode-trapping fungi against nematodes.
登录
查看更多内容
影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
影响因子：
10.7
作者：
Ding, KY;Zhou, K;Shen, Y
通讯作者：
Shen, Y
影响因子：
5.5
作者：
Fargues, J;Smits, N;Quimby, P
通讯作者：
Quimby, P
影响因子：
2.8
作者：
Ahman, J;Ek, B;Tunlid, A
通讯作者：
Tunlid, A
影响因子：
3.5
作者：
Bagga, S;Hu, G;St Leger, RJ
通讯作者：
St Leger, RJ