Enhanced super-resolution microscopy by combined Airyscan and Quantum-Dot-Triexciton Imaging

Enhanced super-resolution microscopy by combined Airyscan and Quantum-Dot-Triexciton Imaging
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通过结合 Airyscan 和量子点三激子成像增强超分辨率显微镜

DOI:
10.1101/2020.05.07.082222
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发表时间:
2020
期刊:
bioRxiv
影响因子:
--
通讯作者:
Manstein
Manstein
中科院分区:
--
文献类型:
--
作者:
Hennig;Manstein

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超分辨率荧光成像提供了关于亚细胞结构的组成、组织和动力学的关键改进信息。量子点三激子成像(QDTI)已被引入作为一种易于使用的子衍射成像方法,实现了几乎2倍的分辨率提高时,与传统的共焦显微镜使用。在这里,我们报告了一个整体的横向和轴向分辨率增加了3倍,与标准的共聚焦显微镜相结合的QDTI与Airyscan的方法。
Super-resolution fluorescence imaging provides critically improved information about the composition, organization and dynamics of sub-cellular structures. Quantum-Dot-Triexciton Imaging (QDTI) has been introduced as an easy-to-use sub-diffraction imaging method that achieves an almost 2-fold improvement in resolution when used with conventional confocal microscopes. Here we report an overall 3-fold increase in lateral and axial resolution compared to standard confocal microscopes by combining QDTI with the Airyscan approach.
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