Wheat Elongator Subunit 4 Negatively Regulates Freezing Tolerance by Regulating Ethylene Accumulation.
Wheat Elongator Subunit 4 Negatively Regulates Freezing Tolerance by Regulating Ethylene Accumulation.
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小麦延伸子4亚基通过调节乙烯积累负调控抗寒性
DOI：
10.3390/ijms23147634
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发表时间：
2022-07-11
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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冰冻胁迫是限制温带作物生产和地理分布的主要因素。拟南芥（Arabidopsis thaliana）是一个具有组蛋白乙酰转移酶活性的6亚基复合体，参与植物发育和防御反应。然而，在植物中，伸长体是否以及如何对冰冻胁迫作出反应尚不清楚。本研究发现，小麦伸长亚单位4 （TaELP4）通过乙烯信号负调控抗冻性。TaELP4启动子含有冷响应元件，在冰冻胁迫下表达上调。亚细胞定位表明，TaELP4和AtELP4定位于细胞质和细胞核。在低温胁迫下，通过bsmv介导的vgs方法沉默TaELP4可显著提高小麦分蘖成活率，而异位表达TaELP4在拟南芥中与Col-0相比，可增加叶片损伤和成活率。进一步的结果表明，TaELP4正调控ACS2和ACS6转录本，这是乙烯生物合成的两个主要限制酶。乙烯含量测定结果表明，TaELP4过表达导致低温胁迫下的乙烯积累量大于Col-0。表观遗传学研究表明，拟南芥AtACS2和AtACS6编码/启动子区组蛋白H3K9/14ac水平显著升高。RT-qPCR结果显示，低温胁迫下EIN2/EIN3/EIL1-CBFs-COR通路受TaELP4调控。综上所述，我们的研究结果表明，TaELP4通过提高ACS2和ACS6的组蛋白乙酰化水平，增加它们的转录和乙烯积累，从而负向调节植物对冰冻胁迫的反应。
Freezing stress is a major factor limiting production and geographical distribution of temperate crops. Elongator is a six subunit complex with histone acetyl-transferase activity and is involved in plant development and defense responses in Arabidopsis thaliana. However, it is unknown whether and how an elongator responds to freezing stress in plants. In this study, we found that wheat elongator subunit 4 (TaELP4) negatively regulates freezing tolerance through ethylene signaling. TaELP4 promoter contained cold response elements and was up-regulated in freezing stress. Subcellular localization showed that TaELP4 and AtELP4 localized in the cytoplasm and nucleus. Silencing of TaELP4 in wheat with BSMV-mediated VIGS approach significantly elevated tiller survival rate compared to control under freezing stress, but ectopic expression of TaELP4 in Arabidopsis increased leaf damage and survival rate compared with Col-0. Further results showed that TaELP4 positively regulated ACS2 and ACS6 transcripts, two main limiting enzymes in ethylene biosynthesis. The determination of ethylene content showed that TaELP4 overexpression resulted in more ethylene accumulated than Col-0 under freezing stress. Epigenetic research showed that histone H3K9/14ac levels significantly increased in coding/promoter regions of AtACS2 and AtACS6 in Arabidopsis. RT-qPCR assays showed that the EIN2/EIN3/EIL1-CBFs-COR pathway was regulated by TaELP4 under freezing stress. Taken together, our results suggest that TaELP4 negatively regulated plant responses to freezing stress via heightening histone acetylation levels of ACS2 and ACS6 and increasing their transcription and ethylene accumulation.
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影响因子：
4.2
作者：
Li, Xiangnan;Cai, Jian;Jiang, Dong
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