Use of postmortem human dura mater and scalp for deriving human fibroblast cultures.
Use of postmortem human dura mater and scalp for deriving human fibroblast cultures.
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DOI：
10.1371/journal.pone.0045282
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Hyde TM
中科院分区：
文献类型：
--
作者：
Bliss LA;Sams MR;Deep-Soboslay A;Ren-Patterson R;Jaffe AE;Chenoweth JG;Jaishankar A;Kleinman JE;Hyde TM
成纤维细胞可以从死亡的个体中收集，在培养物中生长，重编程为诱导多能干细胞（iPSC），然后分化为多种细胞类型，包括神经元。过去的研究已经从动物或人类受试者的体细胞活检中产生了iPSC。以前，只有两项研究成功地从死后的人皮肤中培养成纤维细胞。在这里，我们提出的数据成纤维细胞培养产生的146头皮和/或53硬脑膜样本从146死后人脑捐助者。在我们的总体样本中，硬脑膜培养成功的几率几乎是头皮培养的两倍（OR = 1.95，95% CI：[1.01，3.9]，p = 0.047）。 在两种组织均可用于培养的受试者中使用配对设计（n = 53），硬脑膜培养的成功几率是头皮培养的16倍（OR = 16.0，95% CI：[2.1-120.6]，p = 0.0007）。 无人照料的死亡、组织捐赠来源、较长的死后间隔（PMI）和较高的体重指数（BMI）与头皮培养不成功相关（所有p<0.05），但与硬脑膜无关。虽然头皮细胞比硬脑膜细胞增殖更多且生长更快[F（1，46）= 12.94，p<0.008]，但两种组织都可以作为成纤维细胞系产生和维持。 使用随机的四个病例样本，我们发现死后头皮和硬脑膜都可以成功地重新编程为iPSC细胞系。我们的研究表明，死后的硬脑膜，在较小程度上，头皮，是活的成纤维细胞的可行来源，可用于培养产生iPSC。这些组织可以通过现有的脑组织收集来获取，这对于研究神经精神疾病等疾病至关重要。
Fibroblasts can be collected from deceased individuals, grown in culture, reprogrammed into induced pluripotent stem cells (iPSCs), and then differentiated into a multitude of cell types, including neurons. Past studies have generated iPSCs from somatic cell biopsies from either animal or human subjects. Previously, fibroblasts have only been successfully cultured from postmortem human skin in two studies. Here we present data on fibroblast cell cultures generated from 146 scalp and/or 53 dura mater samples from 146 postmortem human brain donors. In our overall sample, the odds of successful dural culture was almost two-fold compared with scalp (OR = 1.95, 95% CI: [1.01, 3.9], p = 0.047). Using a paired design within subjects for whom both tissues were available for culture (n = 53), the odds of success for culture in dura was 16-fold as compared to scalp (OR = 16.0, 95% CI: [2.1–120.6], p = 0.0007). Unattended death, tissue donation source, longer postmortem interval (PMI), and higher body mass index (BMI) were associated with unsuccessful culture in scalp (all p<0.05), but not in dura. While scalp cells proliferated more and grew more rapidly than dura cells [F (1, 46) = 12.94, p<0.008], both tissues could be generated and maintained as fibroblast cell lines. Using a random sample of four cases, we found that both postmortem scalp and dura could be successfully reprogrammed into iPSC lines. Our study demonstrates that postmortem dura mater, and to a lesser extent, scalp, are viable sources of living fibroblasts for culture that can be used to generate iPSCs. These tissues may be accessible through existing brain tissue collections, which is critical for studying disorders such as neuropsychiatric diseases.
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影响因子：
2.5
作者：
Hjelm BE;Rosenberg JB;Szelinger S;Sue LI;Beach TG;Huentelman MJ;Craig DW
通讯作者：
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DOI：
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发表时间：
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