Laminin-1 Peptides Conjugated to Fibrin Hydrogels Promote Salivary Gland Regeneration in Irradiated Mouse Submandibular Glands.
Laminin-1 Peptides Conjugated to Fibrin Hydrogels Promote Salivary Gland Regeneration in Irradiated Mouse Submandibular Glands.
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DOI：
10.3389/fbioe.2021.729180
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发表时间：
2021
影响因子：
5.7
通讯作者：
Baker OJ
中科院分区：
文献类型：
--
作者：
Nam K;Dos Santos HT;Maslow F;Trump BG;Lei P;Andreadis ST;Baker OJ
先前的研究表明，纤维蛋白水凝胶与Laminin-1肽的化学偶联可以促进唾液腺的形态发生和分化。具体来说，与纤维蛋白化学结合的Laminin-1肽（A99: CGGALRGDN-amide和YIGSR: CGGADPGYIGSRGAA-amide）在体外（例如，使用类器官）和体内（例如，在受伤小鼠模型中）促进了新组织唾液上皮的形成。虽然这些研究是成功的，但该模型在放射治疗后诱导再生模式的有用性仍然未知。因此，本研究的目的是确定经皮注射与纤维蛋白水凝胶化学偶联的Laminin-1肽A99和YIGSR是否促进辐照唾液腺的组织再生。结果表明，经皮注射到经辐照的唾液腺后，A99和YIGSR与纤维蛋白水凝胶化学偶联，促进了功能性唾液腺组织的形成。相反，如果不进行治疗，受辐射的唾液腺会在结构和功能上出现损失。综上所述，这些研究表明含有Laminin-1肽的纤维蛋白水凝胶可植入支架可促进放射唾液腺的分泌功能。
Previous studies demonstrated that salivary gland morphogenesis and differentiation are enhanced by modification of fibrin hydrogels chemically conjugated to Laminin-1 peptides. Specifically, Laminin-1 peptides (A99: CGGALRGDN-amide and YIGSR: CGGADPGYIGSRGAA-amide) chemically conjugated to fibrin promoted formation of newly organized salivary epithelium both in vitro (e.g., using organoids) and in vivo (e.g., in a wounded mouse model). While these studies were successful, the model’s usefulness for inducing regenerative patterns after radiation therapy remains unknown. Therefore, the goal of the current study was to determine whether transdermal injection with the Laminin-1 peptides A99 and YIGSR chemically conjugated to fibrin hydrogels promotes tissue regeneration in irradiated salivary glands. Results indicate that A99 and YIGSR chemically conjugated to fibrin hydrogels promote formation of functional salivary tissue when transdermally injected to irradiated salivary glands. In contrast, when left untreated, irradiated salivary glands display a loss in structure and functionality. Together, these studies indicate that fibrin hydrogel-based implantable scaffolds containing Laminin-1 peptides promote secretory function of irradiated salivary glands.
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影响因子：
2.4
作者：
Braga, M. A.;Tarzia, O.;Groppo, F. C.
通讯作者：
Groppo, F. C.
DOI：
10.1073/pnas.1210662109
发表时间：
2012-11-20
影响因子：
11.1
作者：
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3.8
作者：
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Limesand KH
影响因子：
14
作者：
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通讯作者：
Larsen, Melinda