Measuring changes in tactile sensitivity in the hind paw of mice using an electronic von Frey apparatus.
Measuring changes in tactile sensitivity in the hind paw of mice using an electronic von Frey apparatus.
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DOI：
10.3791/51212
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发表时间：
2013-12-19
期刊：
影响因子：
--
通讯作者：
Chatterjea D
中科院分区：
文献类型：
--
作者：
Martinov T;Mack M;Sykes A;Chatterjea D
测量炎症诱导的机械压力下后爪缩回阈值的变化是评估啮齿动物疼痛感知变化的有用技术。退缩阈值可以首先在基线处测量，然后在药物、毒液、损伤、过敏原或通过在皮肤的非常特定的区域上施加精确的力而诱发的炎症之后测量。电子von Frey装置允许精确评估小鼠后爪缩回阈值，其不受与经典von Frey测量相反的可用细丝尺寸的限制。测量的简易性和快速性允许在快速发作的炎性和神经性疼痛的不同模型中纳入触觉敏感性结果的评估，因为可以在短时间内进行多次测量。可以针对个体基线响应对个体啮齿动物受试者的实验测量进行内部控制，并且容易建立排除标准以标准化实验组内和实验组间的基线响应。因此，使用电子von Frey装置的测量代表了对啮齿动物机械异常性疼痛的成熟的经典von Frey基于顺应性的测定的有用修改，其也可以应用于其他非人哺乳动物模型。
Measuring inflammation-induced changes in thresholds of hind paw withdrawal from mechanical pressure is a useful technique to assess changes in pain perception in rodents. Withdrawal thresholds can be measured first at baseline and then following drug, venom, injury, allergen, or otherwise evoked inflammation by applying an accurate force on very specific areas of the skin. An electronic von Frey apparatus allows precise assessment of mouse hind paw withdrawal thresholds that are not limited by the available filament sizes in contrast to classical von Frey measurements. The ease and rapidity of measurements allow for incorporation of assessment of tactile sensitivity outcomes in diverse models of rapid-onset inflammatory and neuropathic pain as multiple measurements can be taken within a short time period. Experimental measurements for individual rodent subjects can be internally controlled against individual baseline responses and exclusion criteria easily established to standardize baseline responses within and across experimental groups. Thus, measurements using an electronic von Frey apparatus represent a useful modification of the well-established classical von Frey filament-based assays for rodent mechanical allodynia that may also be applied to other nonhuman mammalian models.
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影响因子：
5.5
作者：
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影响因子：
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通讯作者：
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DOI：
10.1590/s0100-879x2004000300018
发表时间：
2004-03-01
影响因子：
2.3
作者：
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通讯作者：
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