Generation and Application of Monoclonal Antibodies Specific for the Envelope Protein pORF132 of Cyprinid Herpesvirus 3
Generation and Application of Monoclonal Antibodies Specific for the Envelope Protein pORF132 of Cyprinid Herpesvirus 3
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鲤科疱疹病毒3型包膜蛋白pORF132特异性单克隆抗体的制备及应用
DOI：
10.3147/jsfp.51.15
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Hao Ke
中科院分区：
文献类型：
--
作者：
Zhenxing Liu;Le Hao;Yanping Ma;Zhiling Liang;Jiangyao Ma;Hao Ke
鲤疱疹病毒3型（CyHV-3）又称锦鲤疱疹病毒（KHV），是锦鲤和鲤鱼的致死性病原。本文报道了抗CyHV-3包膜蛋白pORF 132单克隆抗体的制备方法及其应用。构建了3个重组质粒，分别表达1个完整的pORF 132和2个截短的pORF 132。经诱导后，截短的pORF 132以可溶性形式成功表达，纯化的重组蛋白用于制备单克隆抗体。获得两个杂交瘤细胞克隆，4 E11和4 B11，mAb 4 E11与CyHV-3感染的细胞反应，而不与CyHV-2（金鱼造血坏死病毒）和IcHV-1（癍点叉尾鱼病毒）感染的细胞反应。初步建立了单克隆抗体4 E11与抗CyHV-3 IgY的抗原捕获ELISA方法。该抗原捕获ELISA可区分CyHV-3感染和未感染的鱼样品
Cyprinid herpesvirus 3 (CyHV-3), also named koi herpesvirus (KHV), is a lethal pathogen of koi and common carp Cyprinus carpio. In the present study, we described a procedure to generate.monoclonal antibodies (mAbs) against the envelope protein pORF132 of CyHV-3 and application of the mAb. Three recombinant plasmids targeting the expression of one complete pORF132 and two truncated pORF132 were constructed, respectively. After induction, a truncated pORF132 was successfully expressed in a soluble form and the purified recombinant protein was used in the preparation of mAbs. Two hybridoma cell clones, 4E11 and 4B11, were obtained and mAb 4E11 reacted to CyHV-3 infected cells and not to CyHV-2 (goldfish hematopoietic necrosis virus) and IcHV-1(channel catfish virus) infected cells. An antigen-capture ELISA based on the pair of mAb 4E11 and anti-CyHV-3 IgY was primarily constructed. This antigen-capture ELISA could distinguish between the CyHV-3 infected and uninfected fish samples
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影响因子：
2.6
作者：
C. Tu;Yi-Ping Lu;C. Hsieh;Su-Ming Huang;Shao-Kuang Chang;Meei-Mei Chen
通讯作者：
C. Tu;Yi-Ping Lu;C. Hsieh;Su-Ming Huang;Shao-Kuang Chang;Meei-Mei Chen
影响因子：
3.1
作者：
Jia R;Cheng A;Wang M;Qi X;Zhu D;Ge H;Luo Q;Liu F;Guo Y;Chen X
通讯作者：
Chen X
影响因子：
0.6
作者：
Yuasa, Kei;Sano, Motohiko;Oseko, Norihisa
通讯作者：
Oseko, Norihisa
DOI：
10.1145/1178477.1178570
发表时间：
2006
影响因子：
--
作者：
通讯作者：
--
影响因子：
0.6
作者：
Manabu Yoshino;Hajime Watari;T. Kojima;Masanari Ikedo
通讯作者：
Manabu Yoshino;Hajime Watari;T. Kojima;Masanari Ikedo