Development and evaluation of an antigen-capture ELISA for detection of the UL24 antigen of the duck enteritis virus, based on a polyclonal antibody against the UL24 expression protein.
Development and evaluation of an antigen-capture ELISA for detection of the UL24 antigen of the duck enteritis virus, based on a polyclonal antibody against the UL24 expression protein.
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DOI：
10.1016/j.jviromet.2009.05.011
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发表时间：
2009-10
影响因子：
3.1
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Jia R;Cheng A;Wang M;Qi X;Zhu D;Ge H;Luo Q;Liu F;Guo Y;Chen X
建立了鸭肠炎病毒（DEV）UL 24抗原的抗原捕获酶联免疫吸附试验（AC-ELISA）方法。将表达的UL 24重组蛋白分别免疫鸭和家兔。从鸭和兔中纯化抗UL 24的IgG抗体并用作捕获抗体。用DEV、鸭肝炎病毒（DHV）、鸭B肝炎病毒（DHBV）、小鹅瘟病毒（GPV）、鸭疫里默氏杆菌（R.A.）大肠杆菌（E.大肠杆菌）、多杀性巴氏杆菌（PM）和肠道沙门氏菌（S. E.）。仅DEV标本产生特异性强信号。该方法检测DEV的灵敏度为46 ng/100 μl。与PCR和病毒分离法比较，对实验感染血清的检测符合率为100%。用中和试验和AC-ELISA对临床标本进行对比试验，AC-ELISA的真阳性率和真阴性率分别为0.90和0.67。结果表明，AC-ELISA方法是一种快速、灵敏、可靠的DEV抗原特异性检测方法。
An antigen-capture enzyme-linked immunosorbent assay (AC-ELISA) method was developed for the efficient detection of the UL24 antigen of the duck enteritis virus (DEV) using polyclonal antibodies. Ducks and rabbits were immunized, respectively, with expressed UL24 recombinant protein. The IgG antibodies against UL24 from ducks and rabbits were purified and used as the capture antibodies. The specificity of the optimized AC-ELISA was evaluated by use of DEV, duck hepatitis virus (DHV), duck hepatitis B virus (DHBV), gosling plague virus (GPV), Riemerella anatipestifer (R.A.), Escherichia coli (E. coli), Pasteurella multocida (P.M.) and Salmonella Enteritidis (S.E.). Only DEV specimens yielded a specific and strong signal. The limit of the sensitivity of this method for the detection of DEV was 46 ng/100 μl. Compared with PCR and virus isolation, the rate of agreement for the detection of experimentally infected sera was 100%. A comparative test used on clinical specimens between the neutralization test and the AC-ELISA showed that the proportions of true positives and true negatives by the AC-ELISA were 0.90 and 0.67 respectively. These results indicated that the AC-ELISA approach is rapid, sensitive, and reliable for specific detection of DEV antigen.
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影响因子：
9.4
作者：
Che, XY;Qiu, LW;Yuen, KY
通讯作者：
Yuen, KY
影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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DOI：
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发表时间：
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影响因子：
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作者：
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通讯作者：
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作者：
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