General method for regulating protein stability with light.

General method for regulating protein stability with light.
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DOI:
10.1021/cb400755b
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发表时间:
2014-01-17
影响因子:
4
通讯作者:
Wandless, Thomas J.
Wandless, Thomas J.
中科院分区:
生物学2区
文献类型:
--
作者:
Bonger, Kimberly M.;Rakhit, Rishi;Payumo, Alexander Y.;Chen, James K.;Wandless, Thomas J.

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Posttranslational regulation of protein abundance in cells is a powerful tool for studying protein function. We here describe a novel genetically encoded protein domain that is degraded upon exposure to non-toxic blue light. We demonstrate that fusion proteins containing this domain are rapidly degraded in cultured cells and in zebrafish upon illumination.
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