The Detection of MAPK Signaling
The Detection of MAPK Signaling
复制标题
MAPK信号的检测
DOI：
--
复制
发表时间：
2006
影响因子：
--
通讯作者：
R. Seger
中科院分区：
文献类型：
--
作者：
Y. Shaul;R. Seger
Mitogen‐activated protein kinase (MAPK) cascades are central pathways that participate in the intracellular transmission of extracellular signals. Each of the MAPK signaling cascades seems to consist of three to five tiers of protein kinases that sequentially activate each other by phosphorylation. Since the majority of MAPK cascade components are kinases, the methods used to detect their activation involve determining phosphorylation state and protein kinase activities. The primary method describes the use of immunoblotting with specific anti‐phospho antibody to detect activation of MAPK components. Alternative methods described are immunoprecipitation of desired protein kinases followed by phosphorylation of specific substrates and the use of an in‐gel kinase assay. These methods have proven useful in the study of the MAPK signaling cascades.
登录
查看更多内容
影响因子：
56.9
作者：
HAN, J;LEE, JD;ULEVITCH, RJ
通讯作者：
ULEVITCH, RJ
DOI：
10.1073/pnas.94.14.7337
发表时间：
1997-07-08
影响因子：
11.1
作者：
Tournier, C;Whitmarsh, AJ;Davis, RJ
通讯作者：
Davis, RJ
DOI：
10.1073/pnas.94.18.9687
发表时间：
1997-09-02
影响因子：
11.1
作者：
Diener, K;Wang, XHS;Yao, ZB
通讯作者：
Yao, ZB
影响因子：
56.9
作者：
Chow, CW;Rincon, M;Davis, RJ
通讯作者：
Davis, RJ
从表皮生长因子刺激的 A431 细胞中纯化和表征丝裂原激活的蛋白激酶激活剂。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Seger,R;Ahn,NG;Posada,J;Munar,ES;Jensen,AM;Cooper,JA;Cobb,MH;Krebs,EG
通讯作者：
Krebs,EG