Sour taste responses in mice lacking PKD channels.
Sour taste responses in mice lacking PKD channels.
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DOI：
10.1371/journal.pone.0020007
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Ninomiya Y
中科院分区：
文献类型：
--
作者：
Horio N;Yoshida R;Yasumatsu K;Yanagawa Y;Ishimaru Y;Matsunami H;Ninomiya Y
多囊肾样离子通道 PKD2L1 及其相关伙伴 PKD1L3 是酸味受体的潜在候选者。 PKD2L1 在对酸味刺激做出反应的 III 型味觉细胞中表达，表达 PKD2L1 的细胞的基因消除显着降低了鼓索神经对酸味刺激的反应。然而，PKD2L1 和 PKD1L3 对酸味反应的贡献仍不清楚。我们制作了缺乏 PKD2L1 和/或 PKD1L3 基因的小鼠，并研究了鼓索或舌咽神经对味觉刺激的整个神经反应以及 III 型味觉细胞的味觉反应。在缺乏 PKD2L1 基因的小鼠中，与野生型小鼠相比，鼓索神经对酸味、咸味、苦味和鲜味促味剂的反应降低了 25-45%。相比之下，PKD1L3敲除小鼠的鼓索神经反应以及单敲除和双敲除小鼠的舌咽神经反应与野生型小鼠相似。通过消除 PKD2L1，III 型蕈状味觉细胞（表达 GAD67 的味觉细胞）的酸味反应也降低了 25-45%。这些发现表明 PKD2L1 部分促进了小鼠的酸味反应，并且 PKD 以外的受体也参与了酸味检测。
The polycystic kidney disease-like ion channel PKD2L1 and its associated partner PKD1L3 are potential candidates for sour taste receptors. PKD2L1 is expressed in type III taste cells that respond to sour stimuli and genetic elimination of cells expressing PKD2L1 substantially reduces chorda tympani nerve responses to sour taste stimuli. However, the contribution of PKD2L1 and PKD1L3 to sour taste responses remains unclear. We made mice lacking PKD2L1 and/or PKD1L3 gene and investigated whole nerve responses to taste stimuli in the chorda tympani or the glossopharyngeal nerve and taste responses in type III taste cells. In mice lacking PKD2L1 gene, chorda tympani nerve responses to sour, but not sweet, salty, bitter, and umami tastants were reduced by 25–45% compared with those in wild type mice. In contrast, chorda tympani nerve responses in PKD1L3 knock-out mice and glossopharyngeal nerve responses in single- and double-knock-out mice were similar to those in wild type mice. Sour taste responses of type III fungiform taste cells (GAD67-expressing taste cells) were also reduced by 25–45% by elimination of PKD2L1. These findings suggest that PKD2L1 partly contributes to sour taste responses in mice and that receptors other than PKDs would be involved in sour detection.
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DOI：
10.1073/pnas.1013664107
发表时间：
2010-12-21
影响因子：
11.1
作者：
Chang, Rui B.;Waters, Hang;Liman, Emily R.
通讯作者：
Liman, Emily R.
DOI：
10.1152/ajpheart.1999.277.5.h1669
发表时间：
1999-11-01
影响因子：
4.8
作者：
Kim, Y;Bang, H;Kim, D
通讯作者：
Kim, D
DOI：
10.1152/ajpcell.2001.281.3.c1005
发表时间：
2001-09-01
影响因子：
5.5
作者：
Lyall, V;Alam, RI;DeSimone, JA
通讯作者：
DeSimone, JA
影响因子：
5.3
作者：
DeFazio, RA;Dvoryanchikov, G;Chaudhari, N
通讯作者：
Chaudhari, N
影响因子：
2.4
作者：
Gao N;Lu M;Echeverri F;Laita B;Kalabat D;Williams ME;Hevezi P;Zlotnik A;Moyer BD
通讯作者：
Moyer BD