Voltage-gated sodium channels in taste bud cells.
Voltage-gated sodium channels in taste bud cells.
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DOI：
10.1186/1471-2202-10-20
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发表时间：
2009-03-12
期刊：
影响因子：
2.4
通讯作者：
Moyer BD
中科院分区：
文献类型：
--
作者：
Gao N;Lu M;Echeverri F;Laita B;Kalabat D;Williams ME;Hevezi P;Zlotnik A;Moyer BD
味蕾细胞将有关食物内容物的信息从嵌入顶端微绒毛的味觉感受器传递到支配基侧膜的味觉神经纤维。特别是，味觉细胞去极化，激活电压门控的钠通道，并对味觉作出反应的动作电位。在甜味、苦味和鲜味细胞中，最初的细胞去极化是由于钠通过Trpm5内流，在表达酸味受体PKD2L1的酸味细胞中，通过离子通道未知的阳离子内流。电压门控钠通道感知去极化信号，然后启动动作电位，将味觉信息编码到味觉神经纤维，其分子同一性尚不清楚。我们描述了阳离子通道的分子和组织表达谱，这些阳离子通道参与了电信号从根尖到基侧膜域的传递。Trpm5位于紧密连接的正下方，以接收来自甜、苦和鲜味受体激活的钙信号，而PKD2L1位于味孔。利用激光捕获显微切割收集的小鼠味蕾和舌上皮细胞，在味觉组织中表达了SCN2A、SCN3A和SCN9A电压门控钠通道转录本。SCN2A、SCN3A和SCN9A在味觉细胞亚群的紧密连接下表达。SCN3A和SCN9A在Trpm5细胞中表达，而SCN2A在Trpm5和PKD2L1细胞中表达。HCN4是一个以前与酸味有关的基因，在PKD2L1细胞中表达，并定位于味孔下的细胞突起。SCN2A、SCN3A和SCN9A电压门控钠通道用于感知味觉感受器激活产生的初始去极化信号，并启动味觉细胞动作电位。SCN2A、SCN3A和SCN9A基因产物可能是味觉感受器细胞对河豚毒素敏感的钠电流的原因。
Taste bud cells transmit information regarding the contents of food from taste receptors embedded in apical microvilli to gustatory nerve fibers innervating basolateral membranes. In particular, taste cells depolarize, activate voltage-gated sodium channels, and fire action potentials in response to tastants. Initial cell depolarization is attributable to sodium influx through TRPM5 in sweet, bitter, and umami cells and an undetermined cation influx through an ion channel in sour cells expressing PKD2L1, a candidate sour taste receptor. The molecular identity of the voltage-gated sodium channels that sense depolarizing signals and subsequently initiate action potentials coding taste information to gustatory nerve fibers is unknown. We describe the molecular and histological expression profiles of cation channels involved in electrical signal transmission from apical to basolateral membrane domains. TRPM5 was positioned immediately beneath tight junctions to receive calcium signals originating from sweet, bitter, and umami receptor activation, while PKD2L1 was positioned at the taste pore. Using mouse taste bud and lingual epithelial cells collected by laser capture microdissection, SCN2A, SCN3A, and SCN9A voltage-gated sodium channel transcripts were expressed in taste tissue. SCN2A, SCN3A, and SCN9A were expressed beneath tight junctions in subsets of taste cells. SCN3A and SCN9A were expressed in TRPM5 cells, while SCN2A was expressed in TRPM5 and PKD2L1 cells. HCN4, a gene previously implicated in sour taste, was expressed in PKD2L1 cells and localized to cell processes beneath the taste pore. SCN2A, SCN3A and SCN9A voltage-gated sodium channels are positioned to sense initial depolarizing signals stemming from taste receptor activation and initiate taste cell action potentials. SCN2A, SCN3A and SCN9A gene products likely account for the tetrodotoxin-sensitive sodium currents in taste receptor cells.
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影响因子：
25
作者：
Hiyama, TY;Watanabe, E;Noda, M
通讯作者：
Noda, M
DOI：
10.1159/000093760
发表时间：
2006-01-01
期刊：
TASTE AND SMELL: AN UPDATE
影响因子：
--
作者：
Breslin, Paul A. S.;Huang, Liquan
通讯作者：
Huang, Liquan
影响因子：
2.5
作者：
Chen, YS;Sun, XD;Herness, S
通讯作者：
Herness, S
影响因子：
4.8
作者：
Huang, LQ;Cao, J;Brand, JG
通讯作者：
Brand, JG
影响因子：
56.9
作者：
HECK, GL;MIERSON, S;DESIMONE, JA
通讯作者：
DESIMONE, JA