Induction of inflammation in vascular endothelial cells by metal oxide nanoparticles: effect of particle composition.
Induction of inflammation in vascular endothelial cells by metal oxide nanoparticles: effect of particle composition.
复制标题
金属氧化物纳米颗粒诱导血管内皮细胞炎症：颗粒成分的影响。
DOI：
10.1289/ehp.8497
复制
发表时间：
2007-03
影响因子：
10.4
通讯作者：
Barakat AI
中科院分区：
文献类型：
--
作者：
Gojova A;Guo B;Kota RS;Rutledge JC;Kennedy IM;Barakat AI
暴露于超细颗粒物与心血管疾病发病率增加之间的相关性机制尚不清楚。超细颗粒似乎穿过肺上皮屏障进入血流，增加了与血管内皮细胞直接接触的可能性。由于内皮细胞炎症对心血管病理的发展至关重要，我们假设人主动脉内皮细胞(HAECs)直接暴露于超细颗粒会诱导炎症反应，并且这种反应依赖于颗粒组成。为了验证这一假设，我们用不同浓度(0.001-50μg/mL)的氧化铁(Fe_2O_3)、氧化钇(Y_2O_3)和氧化锌纳米颗粒孵育血管内皮细胞1-8小时，随后检测了这三种炎症标志物细胞内黏附分子-1、白介素8和单核细胞趋化蛋白-1的基因和蛋白水平。我们还利用电感耦合等离子体质谱和透射电子显微镜测定了纳米颗粒与HAECs的相互作用。我们的数据表明，纳米颗粒向HAEC表面的输送和细胞内的摄取与细胞培养液中的颗粒浓度直接相关。所有这三种类型的纳米颗粒都内化到HAECs中，并且经常在细胞内的囊泡中发现。Fe_2O_3纳米颗粒在所测试的任何浓度下都不能在HAECs中引起炎症反应；然而，当阈值浓度超过10μg/mL时，Y2O_2O_3和氧化锌纳米颗粒能够引起显著的炎症反应。在最高浓度下，纳米氧化锌具有细胞毒性，并导致相当大的细胞死亡。这些结果表明，急性暴露于金属氧化物纳米颗粒后，HAECs的炎症反应取决于颗粒组成。
The mechanisms governing the correlation between exposure to ultrafine particles and the increased incidence of cardiovascular disease remain unknown. Ultrafine particles appear to cross the pulmonary epithelial barrier into the bloodstream, raising the possibility of direct contact with the vascular endothelium. Because endothelial inflammation is critical for the development of cardiovascular pathology, we hypothesized that direct exposure of human aortic endothelial cells (HAECs) to ultrafine particles induces an inflammatory response and that this response depends on particle composition. To test the hypothesis, we incubated HAECs for 1–8 hr with different concentrations (0.001–50 μg/mL) of iron oxide (Fe2O3), yttrium oxide (Y2O3), and zinc oxide (ZnO) nanoparticles and subsequently measured mRNA and protein levels of the three inflammatory markers intra-cellular cell adhesion molecule-1, interleukin-8, and monocyte chemotactic protein-1. We also determined nanoparticle interactions with HAECs using inductively coupled plasma mass spectrometry and transmission electron microscopy. Our data indicate that nanoparticle delivery to the HAEC surface and uptake within the cells correlate directly with particle concentration in the cell culture medium. All three types of nanoparticles are internalized into HAECs and are often found within intracellular vesicles. Fe2O3 nanoparticles fail to provoke an inflammatory response in HAECs at any of the concentrations tested; however, Y2O3 and ZnO nanoparticles elicit a pronounced inflammatory response above a threshold concentration of 10 μg/mL. At the highest concentration, ZnO nanoparticles are cytotoxic and lead to considerable cell death. These results demonstrate that inflammation in HAECs following acute exposure to metal oxide nanoparticles depends on particle composition.
登录
查看更多内容
影响因子：
37.8
作者：
Pope, CA;Burnett, RT;Godleski, JJ
通讯作者：
Godleski, JJ
影响因子：
3.4
作者：
Pitsillides, CM;Joe, EK;Lin, CP
通讯作者：
Lin, CP
影响因子：
13.6
作者：
Huggins, FE;Huffman, GP;Robertson, JD
通讯作者：
Robertson, JD
影响因子：
10.4
作者：
通讯作者：
--
动脉粥样硬化的炎症。
DOI：
10.1161/atvbaha.108.179705
发表时间：
2012-09
期刊：
Arteriosclerosis, thrombosis, and vascular biology
影响因子：
--
作者：
Libby P
通讯作者：
Libby P