Rod photoreceptor ribbon synapses in DBA/2J mice show progressive age-related structural changes.
Rod photoreceptor ribbon synapses in DBA/2J mice show progressive age-related structural changes.
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DOI：
10.1371/journal.pone.0044645
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Brandstätter JH
中科院分区：
文献类型：
--
作者：
Fuchs M;Scholz M;Sendelbeck A;Atorf J;Schlegel C;Enz R;Brandstätter JH
DBA/2 J小鼠是青光眼研究中常用的动物模型。DBA/2 J小鼠的眼睛表现出严重的年龄相关性变化，最终导致视网膜神经节细胞和视神经的变性。最近的视网膜电图研究确定了功能缺陷，这表明光感受器细胞也参与了DBA/2 J小鼠视网膜中发生的病理过程。在一项比较研究中，我们研究了DBA/2 J和C57 BL/6对照小鼠视网膜的光学和电子显微镜和PCR分析的解剖和分子变化。在DBA/2 J小鼠的视网膜中，我们发现外丛状层变薄，第一个突触层在视觉信号的传输，和年龄依赖性和进行性退行性结构变化的视杆细胞带状突触。结构带的变化代表了光感受器突触表型，但尚未在该继发性闭角型青光眼动物模型中描述。此外，经典的补体级联反应的基因在老化的DBA/2 J小鼠的感光细胞中上调，这表明带状突触退化和先天免疫系统之间的假定联系。
The DBA/2J mouse is a commonly used animal model in glaucoma research. The eyes of DBA/2J mice show severe age-related changes that finally lead to the degeneration of retinal ganglion cells and the optic nerve. Recent electroretinogram studies identified functional deficits, which suggest that also photoreceptor cells are involved in the pathological processes occurring in the DBA/2J mouse retina. In a comparative study, we examined anatomical and molecular changes in the retinae of DBA/2J and C57BL/6 control mice with light and electron microscopy and with PCR analyses. In the retina of the DBA/2J mouse, we found a thinning of the outer plexiform layer, the first synaptic layer in the transfer of visual signals, and age-dependent and progressive degenerative structural changes at rod photoreceptor ribbon synapses. The structural ribbon changes represent a photoreceptor synaptic phenotype that has not yet been described in this animal model of secondary angle-closure glaucoma. Furthermore, genes of the classical complement cascade were upregulated in the photoreceptor cells of aging DBA/2J mice, suggesting a putative link between ribbon synapse degradation and the innate immune system.
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影响因子：
4.7
作者：
Dyka, FM;May, CA;Enz, R
通讯作者：
Enz, R
影响因子：
4.4
作者：
Panagis, Lambros;Zhao, Xiujun;Danias, John
通讯作者：
Danias, John
视网膜神经节细胞变性是拓扑的，但在DBA/2J小鼠中不是特异性细胞类型。
影响因子：
7.8
作者：
Jakobs, Tatjana C;Libby, Richard T;Ben, Yixin;John, Simon W M;Masland, Richard H
通讯作者：
Masland, Richard H
影响因子：
4.8
作者：
Haeseleer, F;Sokal, I;Palczewski, K
通讯作者：
Palczewski, K
影响因子：
25
作者：
Haeseleer, F;Imanishi, Y;Palczewski, K
通讯作者：
Palczewski, K